Ethanaminium, N-[4-[[4-(diethylamino)phenyl][4-(ethylamino)-1-naphthalenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-, molybdatetungstatephosphate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

The range-finding study was conducted between 2 March 1999 and 6 March 1999 and the definitive study between 10 May 1999 and 14 May 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: LF 961482
- Expiration date of the lot/batch: Not stated
- Purity test date: 05 February 1999

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature in the dark
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: Poorly soluble so WAFs (water accommodated fractions) prepared
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not stated

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: None

Prior to the addition of the test material the dechlorinated tap water was stirred by magnetic stirrer using a stirring rate such that a vortex depth of approximately 25% of the overall height of the water column was achieved. An amount of test material (2.0 g) was added to the vortex of 20 litres of dechlorinated tap water to give the 100 mg/L loading rate. This loading rate was stirred for 23 hours.

The stirring was stopped after 23 hours and the mixture allowed to stand for 1 hour. The WSF was then removed by filtration through 0.2 µm cartridge filters.

The concentration and stability of the test material in the test solutions were verified by chemical analysis at 0, 24 and 96 hours

Analytical monitoring:

yes

Details on sampling:

Water samples were taken from the control and each replicate test vessel at 0, 24 and 96 hours for quantitative analysis. Duplicate samples were taken and stored frozen (approximately -20· C) for further analysis , if necessary. The method of analysis, stability, recovery and test solution analyses are described in the report

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)

-Prior to the addition of the test material the dechlorinated tap water was stirred by magnetic stirrer using a stirring rate such that a vortex depth of approximately 25% of the overall height of the water column was achieved. An amount of test material (2.0 g) was added to the vortex of 20 litres of dechlorinated tap water to give the 100 mg/L loading rate. This loading rate was stirred for 23 hours.

The stirring was stopped after 23 hours and the mixture allowed to stand for 1 hour. The WSF was then removed by filtration through 0.2 µm cartridge filters.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Strain: Oncorhynchus mykiss
- Source: Reputable commercial supplier
- Age at study initiation (mean and range, SD): Juvenile
- Length at study initiation (length definition, mean, range and SD): 4.5 cm (sd = 0.2)
- Weight at study initiation (mean and range, SD): 1.14 g (sd = 0.12
- Method of breeding: N/a
- Maintenance of the brood fish: N/a

ACCLIMATION
- Acclimation period: 30 days
- Acclimation conditions (same as test or not): yes
- Type and amount of food during acclimation: commercial trout pellets discontinued 24h prior to start of definitive study
- Feeding frequency during acclimation: daily
- Health during acclimation (any mortality observed): Health good, ca. 3% mortality prior to start of study

QUARANTINE (wild caught)
- Duration:
- Health/mortality:

FEEDING DURING TEST
- Food type:
- Amount:
- Frequency:

Test type:

semi-static

Water media type:

other: dechlorinated tapwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

207 mg/L total hardness

Test temperature:

14 ± 0.5· C throughout the study

pH:

7.7 - 7.9

Dissolved oxygen:

9.3 - 9.8 mg/L

Nominal and measured concentrations:

WAF's prepared at a loading rate of 100 mg/L.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

NOELR

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test material to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and gave a 96-Hour LLR50* of greater than 100 mg/L loading rate WSF. Correspondingly the No Observed Effect Loading Rate was 100 mg/L loading rate WSF.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L