4-ethylbenzaldehyde

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

July 1992

Deviations:

yes

Remarks:

Tested concentrations, number of fish per concentration and results per concentration not reported

GLP compliance:

not specified

Remarks:

Test conducted for the purpose of scientific research. GLP compliance was not specified.

Specific details on test material used for the study:

- Name of test material cited in publication: EBA
- Source: Sigma Aldrich Hungary (Budapest, Hungary)
- Purity: 98%

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

A stock solution of 1000 mg/L was prepared.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Strain: AB

ACCLIMATION
- Acclimation conditions: The fish were cultured at 25 °C with a pH of 7.0 ± 0.2. The conductivity was 525 ± 50 µS. A photoperiod of 14 hours light / 10 hours dark was maintained.
- Type and frequency of food during acclimation: SDS Small Gran granulated feed (Dietex International Limited Special Diets Services G.B.) (twice every day) and artemia (twice every week).

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

25 °C

pH:

7.0 ± 0.2

Conductivity:

525 ± 50 μS

Nominal and measured concentrations:

Not reported

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: Daily (temperature, pH, dissolved oxygen)
- Solution with test item was changed every 48h

EFFECT PARAMETERS MEASURED: mortality
Number of dead fish were recorded at study termination. Furtermore, sub-lethal effects such as loss of equilibrium, swimming behaviour, respiratory function, pigmentation, etc. were recorded.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

23.49 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Description of key information

The 96-h LC50 was determined to be 23.49 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

23.49 mg/L

Additional information

The short-term toxicity to freshwater fish was determined in a publication according to OECD TG 203. In this study, groups of zebrafish (Danio rerio) were exposed for 96 hours to a series of concentrations under semi-static conditions. Number of dead fish were recorded at study termination. Furtermore, sub-lethal effects such as loss of equilibrium, swimming behaviour, respiratory function, pigmentation, etc. were recorded. Based on the findings, the 96-h LC50 was determined to be 23.49 mg/L