Phenylphosphonic dichloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019-08-23 - 2019-09-26

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

no acclimation period of 48 h was performed

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Phenylphosphonic dichloride

EC Number:

212-534-3

EC Name:

Phenylphosphonic dichloride

Cas Number:

824-72-6

Molecular formula:

C6H5Cl2OP

IUPAC Name:

phenylphosphonic dichloride

Test material form:

liquid

Specific details on test material used for the study:

Name phenylphosphonic dichloride
Batch no. 201 712 01 - 01
Appearance liquid, clear
Composition phenylphosphonic dichloride: > 98 %
CAS No. 824-72-6
EC-No. 212-534-3
Molecular formula C6H5POCI2
Molecular weight 194.98 g/mol
Purity > 98 %
Homogeneity homogeneous

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

The test item was stored in the test facility in a closed vessel at room temperature (16.2 – 23.7 °C) away from humidity, under inert gas, in the dark.
6.1.3 Preparation
The test item is known to react vigorously with water. Therefore, all analyses refer to the reaction product of the test item after contact with water. Due to the reactivity of the test item, during validation the preparation of the stock solution was identified as the crucial step for reproducible concentrations.
A test solution containing 100.0 mg/L test item in dilution water was prepared.
The medium was put into a vessel and the desired amount of test item was pipetted (72.7 µL/L, based on a density of 1.375 mg/µL, stated in the SDS) into the test medium, followed by incubation for 30 min.
Because of the acid character of the test item, the pH of the dilution water was adjusted to 7.8 before filling up to the desired volume. In a non-GPL pre-test, the pH of a test solution with the concentration 100 mg/L was 3.2 and was adjusted to 7.8.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Species Daphnia magna
Authority STRAUS
Strain Berlin
Sex female
Age between 0 and 24 hours
Origin Umweltbundesamt Berlin
In-house breeding since 27. September 2007
Selection of the test system was made following the proposal of the guidelines.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

19.1 – 21.0 °C

pH:

8

Dissolved oxygen:

8.4-8.7 mg/l

Nominal and measured concentrations:

100 mg/l nominal
105-113 mg/l measured

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Nominal Concentration in mg/L	Immobility 24 hours					Immobility 48 hours
	absolute				in %	absolute				in %
Blank control	0	0	0	0	0	0	0	0	0	0
100	0	0	0	0	0	0	0	0	0	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The following results were determined for the test item phenylphosphonic dichloride (species: Daphnia magna).

48h-NOEC ≥ 100 mg/L (nominal)
48h-LOEC > 100 mg/L (nominal)
24h-EC50 > 100 mg/L (nominal)
48h-EC50 > 100 mg/L (nominal)

Executive summary:

One valid experiment was performed.

The study was performed as a limit test using the nominal concentration of 100 mg/L. For the test concentration and the blank control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.

None of the animals was immobilised in the blank control or in the test concentration. Therefore, no statistical evaluation was performed.

Potassium dichromate K₂Cr₂O₇(CAS No. 7778-50-9) was used as positive controlin a current reference study to assure that the test conditions are reliable.

At the beginning and at the end of the test, the content of the test item in the test solutions was determined usingHPLC-UV-determination. The concentration determined at the start of the test was 105 % of the nominal concentration. At the end of the test the determined concentration was 113 % of the nominal concentration. Therefore, the determination of the biological results was based on the nominal concentration (see Guidance Doc. No.23 (§176)).

The following results were determined for the test itemphenylphosphonic dichloride(species:Daphnia magna).

 

48h-NOEC≥100 mg/L (nominal)
48h-LOEC> 100mg/L (nominal)
24h-EC₅₀> 100 mg/L(nominal)
48h-EC₅₀> 100 mg/L(nominal)