1,4,6,10-Dodecatetraen-3-ol,3,7,11-trimethyl-,(4E,6E)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4,6,10-Dodecatetraen-3-ol,3,7,11-trimethyl-,(4E,6E)-

EC Number:

611-799-6

Cas Number:

59121-99-2

Molecular formula:

C15H24O

IUPAC Name:

1,4,6,10-Dodecatetraen-3-ol,3,7,11-trimethyl-,(4E,6E)-

Test material form:

liquid: viscous

Details on test material:

- Name of test material (as cited in study report): Vinylpseudoionol
- Molecular formula (if other than submission substance): C15H24O
- Molecular weight (if other than submission substance): 220.36 g/mol
- Physical state: viscous-liquid
- Analytical purity: 87.1 %
- Impurities (identity and concentrations): 1.2 % water
- Purity test date: not indicated
- Lot/batch No.: Technikumscharge M401
- Limited storage: no
- Stability under test conditions:
- Storage condition of test material: ca. 4 °C, under nitrogen
- Water solubility: poorly water soluble

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The concentration control analyses were performed with test solutions of the following nominal
concentrations (mg/I) of the test substance : 0, 10, 100 mg/L
At the start of the test (after 0 h) samples from vessels without daphnids, at the end of the test (after 48 h) samples from vessels with and without daphnids were analyzed.

Test solutions

Details on test solutions:

Reagents: deionized water (Milli Q-system); acetonitrile (Riedel-de-Haen) ; potassium dihydrogenphosphate (Merck); phosphoric acid 85%(Riedel-de-Haen); reference standard and carrier were delivered by the sponsor.

Sample preparation: For calibration the reference standard was dissolved in acetonitrile ; the stock solutions were diluted with 0.01 M KH2PO4 pH3/acetonitrile (1 /1 v/v) .

Measuring parameters:
Column: Purosper RP-18 5 µm 125 * 3 mm i.d. (Merck)
Mobile phase: 0.01 M KH2PO4 pH3/acetonitrile (38/62 v/v)
Flow rate: 1.0 ml/min
Injection volume: 20 µl
Temperature: ambient
Detection: UV at 290 nm

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain:
- Source : The clone of Dahpnia magna STRAUS 1820 used was supplied by the Instutut National de Recherche Chimique Appliquee, France in 1978. Fron this data on this clone was cultured and bred continously in the Laboratory of Ecology of BASF AG in Ludwigshafen
- Age at study initiation (mean and range, SD): 2 - 24 h- Weight at study initiation (mean and range, SD):
- Feeding during test: none

ACCLIMATION
- Acclimation conditions (same as test or not): yes
- Type and amount of food: green algae
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 - 3.20 mmol/L

Test temperature:

20.3 - 20.7 °C

pH:

7.9 - 8.2

Dissolved oxygen:

7.7 - 8.8 mg/L

Nominal and measured concentrations:

Nominal: 0, 0, 078, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10 mg/L
analysed nominal concentrations (measured at 0 and 48 h) : 0 (< 0.05), 10 (0.07 - 0.16/0.19), 100 (0.71 - 2.07) mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes, nominal 20 ml
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 10 ml fill volume
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h day night regime
- Light intensity: About 2 - 7 µE/(m2~s) at a wave length of 400 - 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility after 0, 3, 6, 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.026 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

For the statistical calculation of the EC50 the probit method (Finney) was used .