Solvent naphtha (coal), xylene-styrene cut

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP status not known, near guideline study, published in peer reviewed literature, minor restrictions in design and/or reporting but otherwise adequate for assessment.

Data source

Reference

Reference Type:

publication

Title:

The toxicological properties of hydrocarbon solvents

Author:

Hine CH, Zuidema HH

Year:

1970

Bibliographic source:

Industrial Medicine 39, 215-200.

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Test substance was applied to intact and abraded skin of 6 shaved rabbits. The application sites were covered in gauze for 24 hours. Skin irritation was assessed using the Draize numerical scoring system at 24 and 72 hours.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

mixed xylenes

IUPAC Name:

mixed xylenes

Constituent 2

Reference substance name:

Xylene

EC Number:

215-535-7

EC Name:

Xylene

Cas Number:

1330-20-7

Molecular formula:

C8H10

IUPAC Name:

xylene

Details on test material:

C-8 aromatics (ortho, meta and para xylene; ethyl benzene; composition not defined). Boiling Range 138-141C.

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

Male rabbits 2-3 kg body weight.

Test system

Type of coverage:

other: gauze

Preparation of test site:

other: shaved, intact and abraded

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

not stated

Duration of treatment / exposure:

not stated

Observation period:

24 and 72 hours

Number of animals:

6

Details on study design:

A maximum of 4 samples was applied to each rabbit.
Comment: guideline methodology would require 4 hour semi-occluded contact.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

primary dermal irritation index (PDII)

Basis:

mean

Score:

2.21

Reversibility:

no data

Remarks on result:

other: mean score for erythema and oedema for both intact and abraded skin

Results 2

Irritation parameter:

erythema score

Remarks on result:

other: mean score for erythema and oedemafor both intact and abraded skin

Irritant / corrosive response data:

All six rabbits were affected on both intact and abraded skin. Individual scores ranged from 1 to 4.

Applicant's summary and conclusion

Interpretation of results:

other: moderately irritating

Remarks:

Criteria used for interpretation of results: other: primary irritation index descriptive rating

Conclusions:

Mixed xylene (C-8 aromatics) was a possible borderline skin irritant in this study.

Executive summary:

Mixed xylenes (C-8 aromatics) were applied to the intact and abraded skin of six shaved rabbits. Application areas were covered with gauze for 24 hours and readings for skin irritation, according to the method of Draize, were made at 24 and 72 hours. Mixed xylenes were found to be moderately irritating under these conditions, with a Primary Dermal Irritation Index of 2.21.