p,p',p''-tris(dimethylamino)trityl alcohol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from computational model developed by USEPA

Justification for type of information:

Data is from computational model developed by USEPA

Qualifier:

according to guideline

Guideline:

other: Modeling database

Principles of method if other than guideline:

ECOSAR estimates log Kow values using the US EPA's KOWWIN Program for aquatic toxicity.Toxicity of a chemical was measured on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program based upon the octanol /water partition coefficient (Kow). Data from ECOSAR class,neutral organics was used.

The estimation methods in EPI Suite™ have been developed by government, academic, and private sector researchers over many years and represent some of the best techniques currently available. The intended application domain of EPI Suite is organic chemicals, and inorganic as well as organometallic chemicals generally can be considered outside the domain.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

Common name: Water flea

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.576 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

no

Conclusions:

On the basis of EPI suite, ECOSAR version 1.11, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 0.576mg/l for the test material in 48 hrs.
Based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified in aquatic acute / chronic 1category as per the criteria mentioned in CLP regulation.

Executive summary:

Based on the prediction done by EPI suite, ECOSAR version 1.11, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted.

On the basis of this program, the LC50 value for short term toxicity to aquatic invertebrates was predicted to be 0.576mg/l for the test chemical in 48 hrs. Therefore, based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified in aquatic acute / chronic 1 category as per the criteria mentioned in CLP regulation.

Description of key information

On the basis of EPI suite, ECOSAR version 1.11, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 0.576mg/l for the test material in 48 hrs. Based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified in aquatic acute / chronic 1 category as per the criteria mentioned in CLP regulation.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.576 mg/L

Additional information

Based on the predicted and experimental data of structurally similar read across chemicals, toxicity of test chemical was determined on the basis of immobility rate of aquatic invertebrates. The studies are summarized below:

First study was based on the prediction done by EPI suite, ECOSAR version 1.11, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted.

On the basis of this program, the LC50 value for short term toxicity to aquatic invertebrates was predicted to be 0.576mg/l for the test chemical in 48 hrs. Therefore, based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified in aquatic acute / chronic 1 category as per the criteria mentioned in CLP regulation.

 

Second and third study was performed from primary source to support the first study for determining the toxicity of test chemical.

Aim of the second study was to assess the short term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilization Test) in a static system for the total exposure period of 48 hrs.

The stock solution 20.0mg/L was prepared by dissolving dark violet liquid in reconstituted water. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted water.

Various concentrations used were 0.06mg/L, 0.12mg/L, 0.24mg/L, 0.50mg/L, 1.00mg/L.

Effects on immobilization were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

The median effective concentration (EC50) for the test substance, on Daphnia magna was determined to be 0.61mg/L on the basis of mobility inhibition effects in a 48 hour study. Thus, based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic acute / chronic 1 category as per the CLP classification criteria.

 

Further the aim of third study was to assess the short term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilization Test) in a static system for the total exposure period of 48 hrs.

The stock solution 20.0 mg/l was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. Various concentrations used were 0.006mg/L, 0.012mg/L, 0.025mg/L, 0.050mg/l, 0.100mg/L. Effects on immobilization were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.037 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic acute / chronic 1 category as per the CLP classification criteria.

Thus, based on the above values, it can be concluded that the chemical is toxic and can be classified under aquatic acute / chronic 1 category.