Resin acids and Rosin acids, ammonium salts

Endpoint summary

Description of key information

Short-term toxicity to fish

On the basis of EPI suite, ECOSAR version 1.1, the LC50 value for short term toxicity to fish was predicted to be 2346.61 mg/l for Resin acids and Rosin acids, ammonium salts in 96 hrs. Based on the LC50 value, it can be concluded that the substance Resin acids and Rosin acids, ammonium salts as not toxic to aquatic environment and thus can be considered “not classified” per the CLP criteria for aquatic environment.

Short-term toxicity to aquatic invertebrates

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic invertebrate predicted for Resin acids and Rosin acids, ammonium salts (CAS: 68649-89-8). Effect concentration i.e EC50 value estimated to be 197.148 mg/l for Daphnia magna for 48 hrs duration. It can be concluded that the Resin acids and Rosin acids, ammonium salts (CAS: 68649-89-8) is likely to be not toxic to aquatic invertebrate, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic algae predicted for Resin acids and Rosin acids, ammonium salts (CAS: 68649-89-8). Effect concentration i.e EC50 value estimated to be 185.95 mg/l for Pseudokirchneriella subcapitata for 72 hrs duration. It can be concluded that the Resin acids and Rosin acids, ammonium salts (CAS: 68649-89-8) is likely to be not toxic to aquatic algae, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

Additional information

Short-term toxicity to fish

Following studies include the predicted data for the target chemical to conclude the toxicity extent of Resin acids and Rosin acids, ammonium salts (CAS: 68649-89-8) towards fish is summarized as follows:

On the basis of EPI suite, ECOSAR version 1.1, the LC50 value for short term toxicity to fish was predicted to be 2346.61 mg/l for Resin acids and Rosin acids, ammonium salts in 96 hrs. Based on the LC50 value, it can be concluded that the substance Resin acids and Rosin acids, ammonium salts as not toxic to aquatic environment and thus can be considered “not classified” per the CLP criteria for aquatic environment.

Similar result is available for the target which is done by usingEPI suite, ECOSAR version 1.1, the LC50 value for short term toxicity to fish was predicted to be 2957.81 mg/l for Resin acids and Rosin acids, ammonium salts in 96 hrs. Based on the LC50 value, it can be concluded that the substance Resin acids and Rosin acids, ammonium salts as not toxic to aquatic environment and thus can be considered “not classified” per the CLP criteria for aquatic environment.

Thus based on the effect concentrations which is in the range 2346.61 mg/l to 2957.81 mg/l give the conclusion that test substance Resin acids and Rosin acids, ammonium salts (CAS: 68649-89) is likely to be non-toxic to aquatic algae at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria.

Short-term toxicity to aquatic invertebrates

Following studies include the predicted data and experimental study for the target chemical and structurally similar read across to conclude the toxicity extent of Resin acids and Rosin acids, ammonium salts (CAS: 68649-89-8) towards aquatic invertebrate is summarized as follows:

 

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic invertebrate predicted for Resin acids and Rosin acids, ammonium salts (CAS: 68649-89-8). Effect concentration i.e EC50 value estimated to be 197.148 mg/l for Daphnia magna for 48 hrs duration. It can be concluded that the Resin acids and Rosin acids, ammonium salts (CAS: 68649-89-8) is likely to be not toxic to aquatic invertebrate, hence it can be considered to be“not classified”as per the CLP classification criteria for aquatic environment.

 

Similar estimation for the target chemical was done by using EPI suite, ECOSAR version 1.1, the LC50 value for short term toxicity to daphnia magna was predicted to be 1357.75 mg/l for Resin acids and Rosin acids, ammonium salts in 48 hrs. Based on the LC50 value, it can be concluded that the substance Resin acids and Rosin acids, ammonium salts as not toxic to aquatic invertebrate and thus can be considered“not classified”per the CLP criteria for aquatic environment.

 

Another estimation of EPI suite, ECOSAR version 1.1, the LC50 value for short term toxicity to Mysid was predicted to be 1914.49 mg/l for Resin acids and Rosin acids, ammonium salts in 48 hrs. Based on the LC50 value, it can be concluded that the substance Resin acids and Rosin acids, ammonium salts as not toxic to aquatic invertebrate and thus can be considered“not classified”per the CLP criteria for aquatic environment.

 

Further the above predicted data of target chemical is supported by the experimental data of structurally similar read across chemical Acetic acid (CAS: 64-19-7) from the publication Journal Water Pollution Control Federation 1965, which suggests that the Short-term toxicity to aquatic invertebrates test was carried out for 4.167 days under static condition to study the effects of Acetic acid on aquatic invertebrate.

The lethal concentration LC50 to 50% of Daphnia magna is 426 mg/l. mortality effect was measured during the test. It can be concluded from the value that the Acetic acid is not toxic to the aquatic invertebrate and can be considered as“not classified”as per classification for aquatic environment.

 

Thus based on the effect concentrations which is in the range 197.148 mg/l to 1914.49 mg/l give the conclusion that test substance Resin acids and Rosin acids, ammonium salts (CAS: 68649-89-8) was likely to be not toxic to aquatic invertebrate at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Following studies include the predicted data and experimental study for the target chemical and structurally similar read across to conclude the toxicity extent of Resin acids and Rosin acids, ammonium salts (CAS: 68649-89-8) towards aquatic algae is summarized as follows:

 

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic algae predicted for Resin acids and Rosin acids, ammonium salts (CAS: 68649-89-8). Effect concentration i.e EC50 value estimated to be 185.95 mg/l for Pseudokirchneriella subcapitata for 72 hrs duration. It can be concluded that the Resin acids and Rosin acids, ammonium salts (CAS: 68649-89-8) is likely to be not toxic to aquatic algae, hence it can be considered to be“not classified”as per the CLP classification criteria for aquatic environment.

 

Another estimation for the target chemical was done by using EPI suite, ECOSAR version 1.1, the LC50 value for Toxicity to aquatic green algae was predicted to be 1093.31 mg/l for Resin acids and Rosin acids, ammonium salts in 48 hrs. Based on the EC50 value, it can be concluded that the substance Resin acids and Rosin acids, ammonium salts as not toxic to aquatic green algae and thus can be considered“not classified”per the CLP criteria for aquatic environment.

 

The above predicted data of target chemical is supported by the experimental data of structurally similar read across chemical Acetic acid (CAS: 64-19-7) from the publication The journal of protozoology 1970, which suggests that the Toxicity to aquatic algae and cyanobacteria test was carried out for 15 mins to study the effects of Acetic acid on aquatic algae. The effective concentration EC100 to 100% of Euglena gracilis for Acetic acid is 720 mg/l. Growth rate was measured during the test. It can be concluded that the Acetic acid is not toxic to the aquatic algae and can be considered as“not classified’as per classification for aquatic environment.

Further it is assissted by the experimental data of structurally similar read across chemical Acetic acid (CAS: 64-19-7) from the ECOTOX database which indicates that the Toxicity to aquatic algae and cyanobacteria test was carried out for 24 hr to study the effects of Acetic acid in aquatic algae.

The effective concentration EC50 to 50% of Chlorococcales is 156 mg/l. Physiology (Assimilation efficiency) effect was measured during the test. It can be concluded for the value that the Acetic acid is not toxic to the aquatic environment and can be considered as“not classified”as per classification for aquatic environment.

 

Thus based on the effect concentrations which is in the range 156 mg/l to 1093.31 mg/l give the conclusion that test substance Resin acids and Rosin acids, ammonium salts (CAS: 68649-89-8) was likely to be not toxic to aquatic algae at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria.