3-(2-ethylhexyloxy)propylamine

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable publication which meets basic scientific principles

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

1988

Reference 2

Reference Type:

publication

Title:

No information

Author:

National Toxicology Program

Year:

1984

Bibliographic source:

http://www.niehs.nih.gov/

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Method: other: nach Haworth, S. et al.: Environ. Mutagen. 5, Suppl. 1, 3-142

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3-(2-ethylhexyloxy)propylamine

EC Number:

226-420-6

EC Name:

3-(2-ethylhexyloxy)propylamine

Cas Number:

5397-31-9

Molecular formula:

C11H25NO

IUPAC Name:

3-[(2-ethylhexyl)oxy]propan-1-amine

Method

Target gene:

his-operon

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA97, TA98, TA100, TA1535

Metabolic activation:

with and without

Metabolic activation system:

S9 mix of Aroclor 1254-induced rat and hamster livers

Test concentrations with justification for top dose:

3, 10, 33, 100, 166, 333, 1000 µg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

9-aminoacridine

sodium azide

other: 4-nitro-o-phenylenediamine; 2-aminoanthracene

Details on test system and experimental conditions:

METHOD OF APPLICATION: preincubation

DURATION
- Preincubation period: 20 minutes
- Exposure duration: 48 hours

NUMBER OF REPLICATIONS: 3

DETERMINATION OF CYTOTOXICITY
- Method: number of his+ colonies, clearing in the density of the background lawn

Evaluation criteria:

A chemical was judged mutagenic (+) or weakly mutagenic (+W) if it produced a reproducible dose-related reponse over the solvent control in replicate trials. A chemical was judged questionable (?) if the results of individual trials were not reproducible, if increases in his + revertants did not meet the criteria for a "+W" response, or if only single dose produced increases in his+ revertants in repeat trials. Chemicals were judged nonmutagenic (-) if they did not meet the criteria for a mutagenic or questionable response.

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA97, TA98, TA100, TA1535

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative