methyl (E)-3-methyl-4-oxo-but-2-enoate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-guideline study, not deviations

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

methyl (2E)-3-methyl-4-oxobut-2-enoate

EC Number:

609-870-1

Cas Number:

40835-18-5

Molecular formula:

C6 H8 O3

IUPAC Name:

methyl (2E)-3-methyl-4-oxobut-2-enoate

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): 3-Formylcrotonsäuremethylester
- Physical state: liquid
- Analytical purity: 99.2 %
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components:
- Lot/batch No.: Abl. Nr. 00-0007
- Storage condition of test material: ambient

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples for analytical determination of the concentration were taken representatively
from each concentration group at start and end of the first and the last interval. Water
samples were taken from the middle of the test vessel using a glass pipette and were
transported to the analytical laboratory in glass ampoules, which were rinsed with test
solution before they were filled with exact volumina.
The transport to the Analytical Laboratory was done on the day of sampling.
For the dates of analysis and storage of samples if applicable see Analytical report in the
Appendix.
The sampels drawn at end of exposure were stored in the freezer for 3 days due to
technical reasons.

Test solutions

Vehicle:

no

Details on test solutions:

The test concentrations were freshly prepared daily separately for each test vessel . The test substance was weighed into a beaker. The defined volume of test water was filled into the test vessel . An aliquot from the test water in the test vessel was taken and mixed with the test substance using an ultra turrax and the mixture was poured into the test vessel and the whole test batch was homogenized again with an ultra turrax.

The test concentrations were prepared as follows: 50, 100, 220, 500 and 1000 g, respecively in 10 L water each.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Zierfisch-Center Klöckner, Ludwigshafen
- Age at study initiation (mean and range, SD): approx. 5 month
- Length at study initiation (length definition, mean, range and SD): 0.65 g (0.40 - 1.19 g)
- Weight at study initiation (mean and range, SD): 4.0 cm (3.6 - 4.7 cm)
- Feeding during test: no


ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Commercial fish diet „Tetramin", supplier Tetra-Werke, Melle, Germany, ad libitum, additionally generally on workdays live brine shrimp (artemia).
- Health during acclimation (any mortality observed):
No signs for sickness, injuries or abnormalities were observed .

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/L = 250 mg/L CaCO3

Test temperature:

22 °C

pH:

7.7 - 8.4

Dissolved oxygen:

5.6 - 8.8 mg/L

Nominal and measured concentrations:

Nominal: 0, 5, 10, 22, 50, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

6.2 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

The median lethal concentration (LC50) after 1, 4, 24, 48, 72 and 96 hours based on the nominal concentrations and based on the mean of the analytically determined concentrations was calculated using probit analysis* . If possible the 95% confidence intervals were given as well .