Registration Dossier
Registration Dossier
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EC number: 609-870-1 | CAS number: 40835-18-5
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study, no deviations
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 14593 (draft)
- Deviations:
- no
- Principles of method if other than guideline:
- Method: other: ISO 14593 (Draft)
- GLP compliance:
- yes
Test material
- Reference substance name:
- methyl (2E)-3-methyl-4-oxobut-2-enoate
- EC Number:
- 609-870-1
- Cas Number:
- 40835-18-5
- Molecular formula:
- C6 H8 O3
- IUPAC Name:
- methyl (2E)-3-methyl-4-oxobut-2-enoate
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report):3-Formylcrotonsäuremethylester
- Molecular formula (if other than submission substance): C6H8O3
- Molecular weight (if other than submission substance): 128 g/mol
- Physical state: liquid
- Analytical purity: 99.2 %
- Impurities (identity and concentrations): not specified
- Lot/batch No.: Abl. Nr. 00-0007
- Stability under test conditions:
- Storage condition of test material: storage at room temperature
- Water solubility: at least 650 mg/L
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Activated sludge from a laboratory wastewater plant treating municipal sewage . Concentration of dry substance 4 mg/I .
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 35 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
- Test temperature: 22 +/- 2 °C
- pH adjusted: no
TEST SYSTEM
- Test baker: glass bottles
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: NaOH
SAMPLING
- Sampling frequency: 0, 7, 14, 21, 28 d
- Sampling method: via CO2 trap
CONTROL AND BLANK SYSTEM
- Inoculum blank: 1
- Abiotic sterile control: 1
- Toxicity control: 1
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- < 10
- Sampling time:
- 0 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 10 - 20
- Sampling time:
- 7 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 40 - 50
- Sampling time:
- 14 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 60 - 70
- Sampling time:
- 21 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 70 - 80
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- 0 d: 0 - 10 %
7 d: 70 - 80 %
Applicant's summary and conclusion
- Interpretation of results:
- other: The test substance is biodegradable under the test conditions (not readily biodegradable according to OECD criteria)
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