Sodium 1-amino-4-[[3,5-bis[[(chloroacetyl)amino]methyl]-2,4,6-trimethylphenyl]amino]-9,10-dihydro-9,10-dioxoanthracene-2-sulphonate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 April 1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 21036/B
Purity: 78 %

Analytical monitoring:

no

Vehicle:

yes

Test organisms (species):

activated sludge

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Nominal and measured concentrations:

40, 128 and 400 mg/l

Reference substance (positive control):

yes

Remarks:

3, 5 - Dichlorophenol

Key result

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Details on results:

No inhibition was seen at any of the test concentrations.

Results with reference substance (positive control):

The IC50 (3h) of 3,5-dichlorophenol was found to be 20 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

The 3h IC50 for FAT 21036/B was found to be greater than 400 mg/L.

Executive summary:

A toxicity study of FAT 21036/B to activated sludge was determined in a 3-hours growth inhibition test according to OECD Guideline 209. The test substance concentrations tested were 40, 128 and 400 mg/l. No inhibition was seen at any of the test concentrations. Hence, the IC50 (3 h) of the test substance FAT 21036/B was found to be >400 mg/L.

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

05 Dec 1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: In house Ciba-Geigy method

Version / remarks:

Comparable to internationally accepted guidelines.

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Name: 21036/A
Purity: 79.9 %

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Reference substance (positive control):

no

Key result

Duration:

21 d

Dose descriptor:

other: BST

Effect conc.:

> 300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Validity criteria fulfilled:

not specified

Conclusions:

The IC50 to microbial population was found to be greater than 300 mg/L.

Executive summary:

A study was performed with FAT 21036/A to determine the toxicity to microorganisms. Based on the findings of the study, the IC50 (BST) of FAT 21036/A was found to be greater than 300 mg/L.

Description of key information

A toxicity study of FAT 21036/B to activated sludge was determined in a 3-hours growth inhibition test according to OECD Guideline 209. The test substance concentrations tested were 40, 128 and 400 mg/l. No inhibition was seen at any of the test concentrations. Hence, the IC50 (3 h) of the test substance FAT 21036/B was found to be >400 mg/L. The findings of the supporting study where the IC50 was found to be >300 mg/L, also support the conclusion that Acid Blue 225 has low toxicity potential for microbial population.

Key value for chemical safety assessment

EC50 for microorganisms:

400 mg/L