Sodium 1-amino-4-[[3,5-bis[[(chloroacetyl)amino]methyl]-2,4,6-trimethylphenyl]amino]-9,10-dihydro-9,10-dioxoanthracene-2-sulphonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 Aug 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: CIBA method A-15

Version / remarks:

Comparable to internationally accepted guidelines.

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 20136/B
Purity: 78 %

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

5 d

Initial conc.:

200 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Remarks on result:

not measured/tested

Key result

Parameter:

BOD5

Value:

20 mg O2/g test mat.

Validity criteria fulfilled:

no

Interpretation of results:

not readily biodegradable

Conclusions:

The BOD5 of FAT 21036/B is 20 mg O2/g test mat.

Executive summary:

The biochemical oxygen demand of FAT 21036/B is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of microorganisms. This test was conducted in accordance with CIBA internal guideline A-15.

Based on the findings of the study, the BOD5 of FAT 21036/B was found to be 20 mg O2/g test mat. In another study, the COD of FAT 20136/B was found to be 1230 mg O2/g, the resulting BOD5/COD ratio is 0.02 suggesting the test substance is not biodegradable.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 Aug 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: CIBA method A-22

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 21036/B
Purity: 78 %

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Parameter followed for biodegradation estimation:

O2 consumption

Remarks on result:

not measured/tested

Key result

Parameter:

COD

Value:

1 230 mg O2/g test mat.

Validity criteria fulfilled:

no

Interpretation of results:

not readily biodegradable

Conclusions:

The COD of FAT 21036/B is 1230 mg O2/g test mat.

Executive summary:

The chemical oxygen demand of FAT 21036/B was determined by measuring the equivalent amount of oxygen in terms of amount of potassium dichromate that is consumed by reacting with the oxidizable components of 1 g of sample material dissolved in 1-liter water. This test was conducted according to CIBA internal guideline A-22.

Based on the findings of the study, the COD of FAT 21036/B was found to be 1230 mg O2/g test mat. In another test, the BOD5 of FAT 21036/B was found to be 20 mg O2/g test material. The resulting BOD5/COD ratio was found to be 0.02 indicating that the substance is not biodegradable.

Reference 3

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 Aug 1988 to 22 Aug 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: CIBA method A - 37

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 21036/B
Purity: 78%

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Duration of test (contact time):

28 d

Initial conc.:

142 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Key result

Parameter:

% degradation (DOC removal)

Value:

22

Sampling time:

28 d

Remarks on result:

other: Standard deviation not available

Validity criteria fulfilled:

no

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

FAT 21036/B fulfils the criteria for inherent, primary biodegradability.

Executive summary:

The inherent biodegradability of FAT 21036/B was determined in a 28 days study according to Zahn wellens test - OECD Guideline for Testing of Chemicals, No. 302B (A-37). The test substance was tested in a concentration of 142 mg/l. Based on the findings of the study, biodegradation after 28 days was 22 %. Hence, the test substance was found to fulfil the criteria for inherent, primary biodegradability.

Description of key information

Acid Blue 225 was found to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable, fulfilling specific criteria

Type of water:

freshwater

Additional information

The biological elimination of FAT 21036/B is found to be 22 % i a Zahn-Wellens test. The chemicxal oxygen demand of FAT 21036/B was found to be 1230 mg O2/g test material whereas the biological oxygen demand was found to be 20 mg O2/g test material.

Several other key studies showed the following results:

COD of 615.50 mg O2/g test material.

BOD5 of 0 mg O2/g test material.

The relevant BOD5/COD quotient is <0.5, therefore, the substance is not indicative of rapid degradation. Taking the data from key study and supporting studies into account, the test substance Acid Blue 225 is considered to be not biodegradable.