Registration Dossier
Registration Dossier
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EC number: 260-480-4 | CAS number: 56966-52-0
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- no
Test material
- Reference substance name:
- 5-chloro-2-(2,4-dichlorophenoxy)aniline
- EC Number:
- 260-480-4
- EC Name:
- 5-chloro-2-(2,4-dichlorophenoxy)aniline
- Cas Number:
- 56966-52-0
- Molecular formula:
- C12H8Cl3NO
- IUPAC Name:
- 5-chloro-2-(2,4-dichlorophenoxy)aniline
- Reference substance name:
- FAT 80023/G
- IUPAC Name:
- FAT 80023/G
- Details on test material:
- - Name of test material (as cited in study report): FAT 80023/G
- Physical state: solid
- Analytical purity: 100%
- Batch No.: ICMD CH 2.23
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: It was not possible to prepare a stable stock solution. So 32, 16, 8 and 4 mg/L respectively of the test substance were weighted directly into the test system
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: activated sludge of a communal sewage treatment plant
- Pretreatment: 3 rimes washing with tap water and addition of synthetic sewage a day before test start
- Initial biomass concentration: 1.44 g/L dw
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 180 min
Test conditions
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.8 (test inititaion)
- Nominal and measured concentrations:
- Nominal test concentrations: control, 4, 8, 16, 32 mg/L
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 180 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- IC50 (180 h) = 16 mg/L
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
