5-chloro-2-(2,4-dichlorophenoxy)aniline

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

5-chloro-2-(2,4-dichlorophenoxy)aniline

EC Number:

260-480-4

EC Name:

5-chloro-2-(2,4-dichlorophenoxy)aniline

Cas Number:

56966-52-0

Molecular formula:

C12H8Cl3NO

IUPAC Name:

5-chloro-2-(2,4-dichlorophenoxy)aniline

Details on test material:

- Name of test material (as cited in study report): FAT 80023/G
- Physical state: solid
- Analytical purity: 100%
- Batch No.: ICMD CH 2.23
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 3 g test substance and 15 mg vehicle were disolved in 200 ml dechlorinated tap water and were heated for 15 min by 50 °C. After that the solution was homogenized for 15 min and added up to 1 L.
- Chemical name of vehicle: Tween 80
- Concentration of vehicle in test medium: 1.1 mg/L in the highest test concentration

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: West Aquarium, Bad Lauterberg, Germany
- Age at study initiation: 122 days
- Length at study initiation (mean): 31 mm
- Weight at study initiation (mean): 0.29 g

ACCLIMATION
- Acclimation period: 33 days
- Acclimation conditions: dechlorinated tap water

- Medical treatment: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

161 mg/L CaCO3

Test temperature:

22.9 ± 0.4 °C

pH:

7.9 - 8.5

Dissolved oxygen:

89 - 98%

Nominal and measured concentrations:

Nominal test concentrations: control, 1.9, 4.3, 9.4, 21, 45, 100, 220 mg/L
Measured test concentrations: < 0.7, n.m., n.m., n.m., n.m., 1.7, 1.4, 1.9 mg/L
n.m. = not measured

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, total volume, test volume: glass aquaria, 5 L, approx. 3 L
- Aeration: slowly aerated
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Intervals of water quality measurement: Temperature, pH, oxygen content after 0, 24, 48, 72, 96 h

OTHER TEST CONDITIONS
- Photoperiod: 12 hours daily
- Light intensity: yellow light

EFFECT PARAMETERS MEASURED: symptoms, mortality after 24, 48, 72, 96 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1.4 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1.9 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

< 1.4 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Details on results:

- Nominal effect concentrations (96 h):
- LC0 = 100 mg/L
- LC50 = 148 mg/L
- LC100 = 220 mg/L
- Behavioural abnormalities: swimming behaviour (slight - severe symptoms at the treatment groups of 45, 100, 220 mg/L over the whole study period
- Mortality of control: none