2,2'-bipyridyl

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1972

Report date:

1972

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

An amount of the test item was applied as aqueous paste to the shorn back of three female rats and covered with an occlusive dressing for 24 h. Subsequently, the dressing was removed, the skin was washed with water and soap and the test item was reapplied 24 h later.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-bipyridyl

EC Number:

206-674-4

EC Name:

2,2'-bipyridyl

Cas Number:

366-18-7

Molecular formula:

C10H8N2

IUPAC Name:

2,2'-bipyridine

Details on test material:

- Name of test material (as cited in study report): 2,2'-bipyridyl
- Substance type: yellowish-fawn crystalline solid
- Physical state: solid

Test animals

Species:

rat

Strain:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 185-195 g

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

water

Remarks:

as aqueous paste

Controls:

not specified

Duration of treatment / exposure:

At least six applications with an exposure time of 24 h each.

Number of animals:

3

Results and discussion

In vivo

Results

Irritation parameter:

other: No scoring system was used

Basis:

other: All animals assessed

Time point:

other: 24h

Reversibility:

no data

Remarks on result:

not determinable because of methodological limitations

Remarks:

No scoring system was used, however, severe erythema were observed after the first 24 h application. After 6 application cycles, also desquamination was observed.

Irritant / corrosive response data:

Severe erythema were observed after the first 24 h application. After 6 application cycles, also desquamination was observed.

Other effects:

Tremors were observed only after the first application. Necropsy and histopathological examination revealed no abnormalities of liver, kidney, thymus, lung, and skin.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

Based on the effects stated in the report, a conclusion on irritating effects of the test item to the skin cannot be drawn due to the significantly prolonged exposure time.

Executive summary:

Skin irritating properties of 2,2'- bipyridine were assessed by applying the test item as aqueous paste to the clipped skin of 3 female rats for 24 h under occlusive dressing. Subsequently, the dressing was removed, the application site was washed and the procedure was repeated. The study neither was performed according to a guideline nor under GLP.

In all rats, severe erythema were observed at the application site after the first application. At the sixth application cycle, desquamination was additionally observed. Tremors were observed only after the first application. Necropsy and histopathological examination revealed no abnormalities.

Based on the effects stated in the report, a conclusion on irritating effects of the test item to the skin cannot be drawn due to the significantly prolonged exposure time. A first evalution occured only after 24 h vs. 4 h according to OECD guideline. Furthermore, the test substance was re- applied for six consecutive days not allowing for an observation period.