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EC number: 206-674-4 | CAS number: 366-18-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Additional toxicological data
Administrative data
- Endpoint:
- additional toxicological information
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- unsuitable test system
- Remarks:
- irrelevant route of exposure.
Data source
Reference
- Reference Type:
- publication
- Title:
- Differential effects of 3 dipyridyl isomers on hepatic microsomal cytochrome P450 and heme oxygenase in rats
- Author:
- Yoshida T, Kobayashi Y, Masuko T, Hashimoto Y, Kuroiwa Y
- Year:
- 1 995
- Bibliographic source:
- Toxicol Lett. 1995, 76(2):145-53; PMID: 7725346
Materials and methods
- Type of study / information:
- Effect of 2,2'-bipyridine on xenobiotic-metabolizing enzymes of rat liver after i.p. administration.
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Male Wistar rats received a single i.p. injection of the test item 2,2'-bipyridine (15.6-125.0 mg/kg bw) dissolved in corn oil and were decapitated 24 h later. Glutathione content was determined from liver which was cut off before liver perfusion was performed. Cytochrome P450 content and activity of different enzymes were measured from liver cell homogenate.
- GLP compliance:
- no
Test material
- Reference substance name:
- 2,2'-bipyridyl
- EC Number:
- 206-674-4
- EC Name:
- 2,2'-bipyridyl
- Cas Number:
- 366-18-7
- Molecular formula:
- C10H8N2
- IUPAC Name:
- 2,2'-bipyridine
- Details on test material:
- - Name of test material (as cited in study report): 2,2'-bipyridyl
Constituent 1
Results and discussion
Any other information on results incl. tables
The test item 2,2'-bipyridine slightly increased rat liver CYP content in the low-dose group (15.6 mg/kg bw) but decreased CYP content with increasing dose levels. In the Western Blot analysis, 2,2'-bipyridine showed no effect neither on CYP 1A1/2 nor on CYP 2B1/2 expression.
The activity of the drug-metabolizing enzymes aminopyrine demethylase, aniline hydroxylase, and dimethylnitrosamine demethylase was slightly but significantly increased at lower dose levels (15.6 -31.2 mg/kg bw) but declined at higher concentrations.
Heme oxygenase activity and GSH content of rat liver was significantly increased in a dose-dependant manner after treatment with 2,2'-bipyridine. Experiments with cycloheximide (an inhibitor of protein biosynthesis) revealed that increased enzyme activity was due to de novo protein synthesis (data not shown).
Table 1: Dose response of 2,2’-bipyridine on drug-metabolizing enzyme activities, heme oxygenase activity and GSH content in rat liver, mean ± SD of 4-6 rats.
Dose [mg/kg] |
Aminopyrine demethylase [nmol/mg protein/min] |
Aniline hydroxylase [nmol/mg protein/min] |
Dimethylnitrosamine demethylase [nmol/mg protein/min] |
Heme oxygenase [nmol/mg protein/h] |
GSH content [µmol/g liver] |
Control |
5.575±0.072 |
1.716±0.015 |
1.782±0.015 |
1.849±0.056 |
4.153±0.283 |
15.6 |
9.501±0.382** |
2.204±0.035** |
2.371±0.148** |
2.141±0.109* |
4.321±0.097 |
31.2 |
8.901±0.722** |
2.200±0.301* |
2.364±0.087** |
3.914±0.268** |
4.543±0.295 |
62.5 |
6.896±0.136** |
1.478±0.050** |
1.349±0.188** |
10.979±0.399** |
7.976±0.189** |
93.7 |
4.336±0.522** |
1.161±0.130** |
1.095±0.007** |
20.945±0.112** |
10.745±0.747** |
124.9 |
3.300±0.082** |
0.838±0.007** |
0.641±0.041** |
27.295±0.545** |
10.657±0.359** |
Applicant's summary and conclusion
- Conclusions:
- Administration of 2,2'-bipyridyl in corn oil to rats via i.p. injection resulted in a concentration-dependant increase of hepatic heme oxygenase activity and glutathione content. The content of cytochrome P450 and of the drug-metabolizing enzymes aminopyrine demethylase, aniline hydroxylase, and dimethylnitrosamine demethylase firstly increased but then declined at higher concentrations.
- Executive summary:
Male Wistar rats received a single i.p. injection of the test item 2,2'-bipyridine (15.6-125.0 mg/kg bw) dissolved in corn oil and were decapitated 24 h later. Glutathione content was determined from liver which was cut off before liver perfusion was performed. Cytochrome P450 content and activity of different enzymes were measured from liver cell homogenate.
Administration of 2,2'-bipyridyl in corn oil to rats via i.p. injection resulted in a concentration-dependant increase of hepatic heme oxygenase activity and glutathione content. The content of cytochrome P450 and of the drug-metabolizing enzymes aminopyrine demethylase, aniline hydroxylase, and dimethylnitrosamine demethylase firstly increased but then declined at higher concentrations.
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