Fatty acids, C16-18 and C18-unsatd., reaction products with diethylenetriamine, di-Me sulfate-quaternized

Long-term toxicity to aquatic invertebrates

Description of key information

The 63d NOEC of >100 μg/L was determined in a 21-day-reproduction test with Daphnia magna over 3 generations. Daphnids were exposed to the effluent of the activated sludge unit containing the degradation products of partially unsaturated IQAC, DMS quaternised.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 µg/L

Additional information

The 21-day-reproduction test of the effluent of a model activated sludge unit fed with 10 mg/L partially unsaturated IQAC (containing 10% of Isopropanol) to Daphnia magna was studied under static renewal conditions. Daphnidswere exposed to the effluent of the activated sludge unit containing the degradation products of the test item. A control activated sludge unit without the test item was run in parallel. The test duration for every generation was 21 days. Neonates of the 14thtest day were exposed as filial generation under the same conditions as the parental generation. Filial generations 1 and 2 were monitored for reproduction rate, time of the first offspring and parental mortality.

Parental generation: In the parental generation there was no difference between the exposed and the not exposed daphnids, however the first offspring in the controls was one day later. Mortality of the parental generation was slightly higher in the control.

 

F1 generation:

42 days after the start of exposition of parental generation, the reproduction rate of the surfactant exposed F1 generation was enhanced and exceeded that of the control. The mean number of parental daphnids was greater than in the control. There was no difference in the time of appearance of the first offspring. Mortality of the parental generation was slightly higher in the control.

 

F2 generation:

63 days after the start of exposition of the first parental generation, in the second filial generation some adverse effects were observed for the surfactant exposed Daphnids: Growth rate decreased and the size and colour of the control animals were not reached. The reproduction rate was significant lower than in the parental generation and the F1 generation. The mortality of the parental animals increased. During the test, partially unsaturated IQAC, DMS quaternised, is eliminated in the model activated sludge unit by > 90%. The elimination is calculated to be 97.75% taking into account the inflow into the model activated sludge unit of the test substance with a concentration of 10 mg/l, the dilution of the effluent of the elimination unit in ratio 1:1.25 and the separately determined EC0=0.1 mg/l to Daphnia magna. Thus a NOEC of >100 μg/L is calculated.

 

The test material contains isopropanol. The toxicity of isopropanol against Daphnia magna is more than 10 000 times lower than the toxicity of the test material.Water solubility of the test item may be slightly enhanced leading to a better bioavailability and slightly enhanced toxicity. But these effects are regarded to have a minor influence on the test result.Therefore it can be concluded in a first approach, neglecting additive effects, that the observed effect values can be attributed to the active ingredient itself.

This study is classified as acceptable and satisfies basic scientifically requirements for a chronic toxicity study with freshwater invertebrates, although not all data were reported.