1-Butanaminium, 4-hydroxy-N,N,N-trimethyl-4-oxo-, inner salt

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study performed under GLP and in accordance with Czechian testing methods (similar to OECD-/EU-testing guidelines)

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Method XI Determination of biodegradability - Activated Sludge Respiration Inhibition test, Regulation of the Ministery of Environment of the Czech republic No. 222/2004, Annex 2. (identical with Method C.11 of Directive 67/548/EEC):

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): 4-butyrobetain (BUBET)
- Molecular formula: C7H15NO2
- Molecular weight: 145.20 g/mol
- Physical state: colourless solution

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The following test item concentrations were tested:
- 100 mg/l
- 180 mg/l
- 320 mg/l
- 560 mg/l
- 1000 mg/l

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Hardness:

not measured

Test temperature:

20°C

pH:

The pH was 7.51-8.0 during the test.

Dissolved oxygen:

not measured

Salinity:

freshwater used

Nominal and measured concentrations:

The following nominal test item concentrations were tested:
- 100 mg/l
- 180 mg/l
- 320 mg/l
- 560 mg/l
- 1000 mg/l

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorphenol

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

The rate of inhibition for the test item ranged from 0 to 4.4%.

Results with reference substance (positive control):

The rate of inhibition for the reference compound ranged from 0 to 83.1%; the IC50 was determined to be 10.1 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The IC50 of the aqueous solution on waste water bacteria was found to be > 1000 mg/l. The IC50 for the pure material was extrapolated to be > 400 mg/l.