Registration Dossier
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EC number: 457-470-9 | CAS number: 407-64-7
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study performed under GLP and in accordance with OECD-/EU-testing guidelines)
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Details on test material:
- - Name of test material (as cited in study report): 4-Trimethylaminobutyrobetain
- Molecular formula: C7H1SN02
- Molecular weight: 145
- Physical state: liquid
- Stability under test conditions: yes
- Storage condition of test material: at room temperature
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, industrial, non-adapted
- Details on inoculum:
- no data available
- Duration of test (contact time):
- 14 d
Initial test substance concentration
- Initial conc.:
- 216 mg/L
- Based on:
- DOC
Reference substance
- Reference substance:
- aniline
Results and discussion
- Preliminary study:
- No pretest performed.
- Test performance:
- - Standard duration of test is 28 days; as the plateau phase was already reached at day 5, the study was terminated after 14 days.
- Adaptationphase: 3 days
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 98
- Sampling time:
- 14 d
- Details on results:
- Points of degradation plot (test substance):
- 69 % degradation after 3 d
- 100 % degradation after 5 d
- 93 % degradation after 7 d
- 97 % degradation after 10 d
- 98 % degradation after 14 d
BOD5 / COD results
- Results with reference substance:
- Points of degradation plot (reference substance):
- 12 % degradation after 3 d
- 97 % degradation after 5 d
- 99 % degradation after 7 d
- 96 % degradation after 10 d
- 97 % degradation after 14 d
Any other information on results incl. tables
- 10-Day window (% DOC): 97%
- Plateau-phase reached after 5 days
- Elimination by adsorption: 9% after 3 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item showed a mean biodegradation of 98% after 14 days.
- Executive summary:
The study was performed with an 40% aqueous solution under GLP in 1995 according to EU-testing method C.4 "Dissolved Organic Carbon (DOC) Die Away Test". A concentration of 216 mg/l DOC was tested. As the plateau phase was already reached after 5 days, the study was stopped after 14 days. Based on the mean biodegradation rate of 98% after 14 days, the test item can be regarded as "readily biodegradable".
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