Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 213-983-8 | CAS number: 1070-89-9
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Information from published industry source (USEPA), limitations in design and/or reporting but otherwise adequate for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 965
- Report date:
- 1965
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Two Sprague-Dawley rats per sex per dose group were exposed by inhalation to the test substance for 4 hours. After an observation period of 14 days, animals were necropsied.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 1,1,1,3,3,3-hexamethyldisilazane
- EC Number:
- 213-668-5
- EC Name:
- 1,1,1,3,3,3-hexamethyldisilazane
- Cas Number:
- 999-97-3
- Molecular formula:
- C6H19NSi2
- IUPAC Name:
- 1,1,1-trimethyl-N-(trimethylsilyl)silanamine
- Details on test material:
- - Name of test material (as cited in study report): Hexamethyldisilazane
- Molecular formula (if other than submission substance): C6H19NSi2
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 250 g
- Housing: individually, stock cages
- Diet: ad libitum, standard laboratory rat diet from Wayne Lab Blox, Allied Mills, Chicago, Illinois
- Water: ad libitum
- Acclimation period: 5 days
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: glass inhalation chamber
- Exposure chamber volume: 5.5 liter
- System of generating vapour: vapours were produced by bubbling dried metered arid thorugh the undiluted test material. Additional dried metered air was mixed with the vapour stream to achieve the desired concentration levels.
POSITIVE CONTROL
- No. of animals: 4 groups of 2 animals per sex per dose
- Positive control compound: 10% ammonium hydroxide solution
- Concentrations: 8.5, 18.9, 33, 72.5 mg/L air - Analytical verification of test atmosphere concentrations:
- not specified
- Duration of exposure:
- ca. 4 h
- Remarks on duration:
- Except 60.5 and 94.3 mg/L dose group, which were exposed 110 and 70 min., resp.
- Concentrations:
- 5.6, 19, 30.4, 60.5, 94.3 mg/L air
- No. of animals per sex per dose:
- 2
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs - Statistics:
- The mortality data were analyzed statistically, employing the technique of Litchfield and Wilcoson (J.T. Litchfield, Jr. and F. Wilcoxon, "A Simplified Method of Evaluating Dose-Effect Experiments." J. Pharm. & Exp. Ther., Vol 96, No. 2, pp. 99-113, 1949.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 10.3 mg/L air
- Based on:
- test mat.
- 95% CL:
- 6.4 - 16.5
- Exp. duration:
- 4 h
- Mortality:
- - No mortality observed in animals exposed to 5.6 mg/L
- All animals exposed to 19, 30.4, 60.5, 94.3 mg/L died, within 70 minutes to 48 hours after exposure - Clinical signs:
- other: - Hypoactivity, hyperpnoea and ataxia were observed in animals exposed to 30.4, 60.5 or 94.3 mg/L, after 4 to 14 minutes of exposure. Sedation was noted at the 10 to 21 minute point. Sedation and hyperpnoea persisted until death. - Hypoactivity was observ
- Gross pathology:
- - Necroscopy revealed mild lung haemorrhage in animals exposed to 19, 30.4, 60.5 or 94.3 mg/L hexamethyldisilazane.
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
