3,5-dimethylhex-1-yn-3-ol

Endpoint summary

Description of key information

The test article is of low acute toxicity, the oral LD50 > 300 mg/kg bw, the dermal LD50 is > 1000 mg/kg bw and the 4 hour aerosol inhalation LC50 is > 4.9 mg/l.  This equals an one hour exposure of 19700 mg/m³ aerosol.

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Reference

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test done before GLP and Guidelines were established.

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

EPA OTS 798.1175 (Acute Oral Toxicity)

GLP compliance:

not specified

Limit test:

yes

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

The outbred Sprague-Dawley rat, weighing 202 to 283 grams, was used for this study. The animals were obtained from Buckshire Corp., Perkasie, PA 18944 (U.S.D.A. License #23-BL) and were nulliparous and non-pregnant.
The animals were housed and maintained in accordance with standards set forth in the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 86-23). The rats were acclimated to the laboratory for at least 5 days prior to dosing and the weight variation did not exceed +/- 20% of the mean weight for each sex.
The animals were individually identified by an ear punch. Each cage was identified with a cage card, displaying the project number, animal
number, sex, date dosed, dose level and responsible technician's initials.

Temperature: 18°C - 26°C (64.4°F - 78.8°F)

Relative Humidity, %: 40 - 70

Light: 12-hour lightldark cycle

Diet: Wayne Rodent-Blox and tap water were provided gd libitum. Based on our current knowledge, no contaminants are known to be in this diet or water which might be expected to interfere with the objectives of the study.

Caging: Stainless steel elevated wire mesh flooring, 5 ratslcage by sex.

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

The test article was administered as supplied, at a dose level of 200 mg/kg.
One group of ten (5 male & 5 female) albino rats was deprived of food but not water overnight prior to dosing. Each animal was weighed and dosed by direct administration of the test article into the stomach by gavage.
Following administration, the animals were allowed food and water ad libitum for the 14-day observation period during which time, the rats were observed for signs of toxicity and mortality. Animals were observed frequently on the day of dosing. A careful clinical examination was performed at least once each day (7 dayslweek). On weekdays, a second observation of mortalitylmoribundity was performed.
Individual weights were recorded on the day of dosing, weekly thereafter, and prior to sacrifice. The animals were euthanized using carbon dioxide at the conclusion of the observation period. Gross necropsies were performed on all animals.

Doses:

200 mg/kg

No. of animals per sex per dose:

5 mal and 5 female

Control animals:

no

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 200 - < 500 mg/kg bw

Based on:

test mat.

Mortality:

none

Gross pathology:

Gross Pathology:

Males
No gross abnormalities were observed for the animals necropsied at the conclusion of the 14-day observation period.

Females
No gross abnormalities were observed for the animals necropsied at the conclusion of the 14-day observation period.

Other findings:

Observations:

Males

Immediate - 5/5 animals appeared normal.
1 hour - 5/5 animals appeared lethargic.
4 hours - 5/5 animals appeared normal.
Day 1 - 1/5 exhibited audible respiration (gasping);
4/5 animals appeared normal.
Day 2-Day 14 - 5/5 animals appeared normal.


Females

Immediate - 5/5 animals appeared normal.
1 hour - 5/5 animals appeared lethargic.
4 hours - 5/5 animals appeared normal.
Day 1 - 1/5 animals exhibited vocalization,
4/5 animals appeared normal.
Day 2-Day 14 - 5/5 animals appeared normal.

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

The test article, when administered as supplied to 5 male and 5 female albino rats, appears to have an acute oral LD50 greater than 200 mg/kg.
Based on the results outlined in this study and considering the 1972 study, the LD0 is 200 mg/kg bw and it is believed that the LD50 is >300 mg/kg bw.

Executive summary:

The test article, when administered as supplied to 5 male and 5 female albino rats, appears to have an acute oral LD50 greater than 200 mg/kg.

Based on the results outlined in this study and considering the 1972 study, the LD0 is 200 mg/kg bw and it is believed that the LD50 is >300 mg/kg bw.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Dose descriptor:

LD50

Value:

300 mg/kg bw

Quality of whole database:

Key study is done before GLP was established. Klimish rating = 2. Two other studies support the result of the key study, which is done according to EPA OTS 798.1175 (Acute Oral Toxicity).

Acute toxicity: via inhalation route

Link to relevant study records

Reference

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1972

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test done before GLP and Guidelines were established.

Qualifier:

no guideline followed

Principles of method if other than guideline:

Single Exposure

A group of ten , male, albino rats weighing between 200 and 500 grams each was used in this study, The rats were placed i n a 70 liter all glass exposure chamber and exposed to a saturated atmosphere of the test material in air for one hour, The material was administered
as an aerosol with particles 3 - 5 microns in diameter. The rate of flow was 23,9 liters per minute at a temperature of 76 °F. The air was passed through a dessicant prior to being passed through the test material.
By differential weighing it was calculated that the rats were subjected to a concentration of 19,700 ppm. This is an average value over the one hour exposure period.

GLP compliance:

no

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

whole body

Vehicle:

air

Duration of exposure:

1 h

Concentrations:

19,700 ppm

No. of animals per sex per dose:

10 male

Control animals:

no

All of the rats survived the one hour exposure period. During the exposure period the rats at first huddled together and their breathing increased in depth. After exposure the rats exhibited complete muscular rigidity with shallow respiration. They remained like this for from one to eight hours and then recovered and were normal throughout the remainder of the observation period. In all rats their eyes appeared normal immediately after removal from the exposure chamber and remained normal throughout the entire observation period. At the end of the 14 day observation period all rats were subjected to gross autopsy examination.

All rats appeared normal on gross examination.

Interpretation of results:

other: non toxic

Remarks:

Criteria used for interpretation of results: OECD GHS

Conclusions:

From the results obtained in this study it appears that Surfynol 61 would be considered non toxic.
The one hour LC50 is greater than 19.7 mg/l. This converts to a four hour LC50 greater than 4.9 mg/l

Executive summary:

From the results obtained in this study it appears that Surfynol 61 would be considered non toxic.

The one hour LC50 is greater than 19.7 mg/l. This converts to a four hour LC50 greater than 4.9 mg/l

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Dose descriptor:

LC50

Value:

19 700 mg/m³ air

Quality of whole database:

Study is from the early 1970ies before GLP and Guidelines were established. Klimish rating = 2.

Acute toxicity: via dermal route

Link to relevant study records

Reference

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1972

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test done before GLP and Guidelines were established.

Qualifier:

no guideline followed

Principles of method if other than guideline:

Single 24 hours exposure

One group of six albino rabbits weighing between 2.0 and 3.0 kg. Each was employed in this study. All animals had their backs clipped free of hair 24 hours prior to testing.
The following dosage level was used:
1000 mg/kg bw
The sample was used as supplied.
All rabbits were weighed and the correct amount of Air Products and Chemicals, Inc., Surfynol 61 was applied to the bare back of each animal, The rabbits were restrained for 24 hours at which time their backs were wiped off. The animals were observed for a fourteen day period for signs of toxicity and for mortalities. Gross autopsies were performed on all animals which died during the 14 day observation period where possible and on all survivors.

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Species:

rabbit

Strain:

other: albino

Sex:

not specified

Type of coverage:

not specified

Vehicle:

unchanged (no vehicle)

Details on study design:

One group of six albino rabbits weighing between 2.0 and 3.0 kg. Each was employed in this study. All animals had their backs clipped free of hair 24 hours prior to testing.
The following dosage level was used:
1000 mg/kg bw
The sample was used as supplied.
All rabbits were weighed and the correct amount of Air Products and Chemicals, Inc., Surfynol 61 was applied to the bare back of each animal, The rabbits were restrained for 24 hours at which time their backs were wiped off. The animals were observed for a fourteen day period for signs of toxicity and for mortalities. Gross autopsies were performed on all animals which died during the 14 day observation period where possible and on all survivors.

Sex:

not specified

Dose descriptor:

LD50

Effect level:

> 1 000 mg/kg bw

Based on:

test mat.

Mortality:

none

Other findings:

One hour after dosing all rabbits exhibited convulsions. All recovered and survived the 14 day observation period.
The skin over the area of administration was brownish red in color and very dry. All organs appeared normal on gross observation.

Interpretation of results:

other: non toxic

Remarks:

Criteria used for interpretation of results: other: Guide to Precautionary Labeling of Hazardous Chemicals, Manual L-1, 7th Edition, 1970 published by the Manufacturing Chemists Association, pages 12-14

Conclusions:

Air Products and Chemicals, Inc., Surfynol 61 as tested in rabbits, has an acute intact dermal LD50 of greater than 1000 mg/kg and would be considered non toxic under the guidelines outlined in Guide to Precautionary Labeling of Hazardous Chemicals, Manual L-1, 7th Edition, 1970 published by the Manufacturing Chemists Association, pages 12-14.

Executive summary:

Air Products and Chemicals, Inc., Surfynol 61 as tested in rabbits, has an acute intact dermal LD50 of greater than 1000 mg/kg and would be considered non toxic under the guidelines outlined in Guide to Precautionary Labeling of Hazardous Chemicals, Manual L-1, 7th Edition, 1970 published by the Manufacturing Chemists Association, pages 12-14.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Dose descriptor:

LD50

Value:

1 000 mg/kg bw

Quality of whole database:

Studies are from the early 1970ies before GLP and Guidelines were established. Klimish rating = 2.

Additional information

Justification for selection of acute toxicity – oral endpoint
Three studies available. The chosen one is the most recent one (1991) is following a guideline (EPA OTS 798.1175), while the other two studies (1971/1972) are less reliable and do not clearly follow a guidline. But both are supporting the result of the selected key study.

Justification for selection of acute toxicity – inhalation endpoint
Only one study available.

Justification for selection of acute toxicity – dermal endpoint
Only one study available.

Justification for classification or non-classification

According to the UN Globally Harmonized System of Classification and Labelling of Chemicals (GHS) Part 3 Chapter 3.1 no classification required for inhalation or dermal toxictiy, for oral toxicity its is considered to be Category 4.