Trimorpholinophosphine oxide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 Jan - 28 May 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Version / remarks:

17 Jul 1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Trimorpholinophosphine oxide

EC Number:

224-662-7

EC Name:

Trimorpholinophosphine oxide

Cas Number:

4441-12-7

Molecular formula:

C12H24N3O4P

IUPAC Name:

trimorpholinophosphine oxide

Test material form:

solid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: mixed microbial population (activated sewage sludge), effluent of municipal sewage treatment plant of the city of Darmstadt (Südhessische Gas und Wasser AG)
- Treatment: Filtration through a coarse folded filter
- Concentration in the reaction mixture: 5 mL/L
- Preparation/Conditioning: Aeration for 5 days

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

1 000 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 22 ± 2 °C

TEST SYSTEM
- The test material was dissolved in a mineral medium and inoculated with a mixed microbial population, and incubated under arrobic conditions in the dark for 28 days. During the incubation period, biodegradation of the test material was determined by the decrease in the concentration of dissolved oxygen in the test material preparation.

SAMPLING
- Sampling frequency: Sampling was performed on Days 0, 7, 14, 21, and 28

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

3

Sampling time:

28 d

Details on results:

Toxicity control: 20.1% degradation after 28 d.

BOD5 / COD results

Results with reference substance:

Degradation of reference item (Aniline) after 28 d: 78%.

Any other information on results incl. tables

Tab 1: Degradation [%] of test item, reference and toxicity control

Time [d]	% degradation
	Test material	Aniline	Toxicity Control
7	4	75	38
14	4	83	43
21	5	70	30
28	3	78	33

The degradation of the reference compound Aniline reached 60% by Day 14, confirming the activity of the inoculum.

The criterion for ready biodegradability of > 60% removal of the ThOD within the 28 d period was not reached. Therefore, the test substance cannot be considered as readily biodegradable.

The degradation of the toxicity control was > 25% (ThOD) and > 35% (DOC) (required pass level) after 14 d.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test item is not readily biodegradable according to the criteria of the OECD guideline 301 D.