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EC number: 216-760-3 | CAS number: 1658-56-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Remarks:
- intraperitoneal
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
- Principles of method if other than guideline:
- 5 male and 5 female NMRI mice are administered 700 mg/kg bw test item by a single intraperitoneal injection to the abdominal cavity. The solution comprises 7 % suspension of the test item in 0.5 % aqueous Carboxymethyl cellulose (CMC). Test animals are monitored for mortality and toxic symptoms for 14 days and surviving animals are subjected to gross pathology analysis at necropsy.
- GLP compliance:
- not specified
- Limit test:
- yes
Test material
- Reference substance name:
- Sodium 4(-2-hydroxy-1-naphthylazo)naphthalenesulphonate
- EC Number:
- 216-760-3
- EC Name:
- Sodium 4(-2-hydroxy-1-naphthylazo)naphthalenesulphonate
- Cas Number:
- 1658-56-6
- Molecular formula:
- C20H13N2NaO4S
- IUPAC Name:
- NA
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Versuchstierzucht WIGA, Sulzfeld, DE
- Weight at study initiation: males: 24.0 g (mean); females: 22.0 g (mean)
- Fasting period before study: 16 hours
- Diet: ad libitum; Herilan MRH-Kraftfutter (H. Eggersmann, Rinteln/Weser, DE)
- Water: ad libitum
ENVIRONMENTAL CONDITIONS
- not specified
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- CMC (carboxymethyl cellulose)
- Details on exposure:
- An aqueous solution of 7 % test item suspended in 0.5 % carboxylmethyl cellulose was administered to each animal by intraperitoneal injection to the abdominal cavity.
- Doses:
- All animals were administered 700 mg/kg bw
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: 1, 24 and 48 hours and 7 and 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: mortality and toxic symptoms
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 700 mg/kg bw
- Based on:
- test mat.
- Mortality:
- 1 hour: no mortality was observed
24 hours: no mortality was observed
48 hours: 3 male and 2 female animals died
7 days: no further mortality was observed
14 days: no further mortality was observed - Clinical signs:
- Clinical signs were nonspecific
- Gross pathology:
- Dead animals: no intra-abdominal test item precipitation or lesions
Sacrificed animals: no intra-abdominal test item precipitation or lesions
Any other information on results incl. tables
Table 1: mortality of test animals administered test item by intraperitoneal injection to the abdominal cavity
Dose (mg/kg bw) | Concentration (%) | Gender | No. animals | Number of animals found dead within: | ||||
1 hour | 24 hours | 48 hours | 7 days | 14 days | ||||
700 | 7 | male | 5 | 0 | 0 | 3 | 3 | 3 |
700 | 7 | female | 5 | 0 | 0 | 2 | 2 | 2 |
Applicant's summary and conclusion
- Conclusions:
- LD50 (intraperitoneal injection, mouse) was found to be approximately 700 mg/kg bw.
- Executive summary:
The acute toxicity of the test item by intraperitoneal injection was evaluated in an experimental study in a limit test without controls. 7 % test item suspended in an aqueous solution of 0.5 % carboxymethyl cellulose was administered to 5 male and 5 female NMRI mice at a concentration of 700 mg/kg bw test item by intraperitoneal injection to the abdominal cavity. Mortality and toxic symptoms were observed at 1, 24 and 48 hours and 7 and 14 days after administration and all animals were subjected to gross pathology analysis at necropsy.
After 1 and 24 hours, no mortality was observed. After 48 hours, 3 male and 2 female animals were found dead. No further mortality occurred within the 14-day study period. Unspecific toxic symptoms observed during the study period. No intra-abdominal test item precipitation or lesions were observed in any test animals at necropsy. The LD50 of the test item was estimated to be approximately 700 mg/kg bw in rats following intraperitoneal injection.
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