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EC number: 217-442-7 | CAS number: 1852-16-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- See the Analogue Approach Report attached in Section 13 of the IUCLID dossier.
- Reason / purpose for cross-reference:
- other: Read across to target substance
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- Diluent: US EPA moderately hard reconstituted
Solvent: None required
Controls: Diluent only - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Strain: Onchorhyncus mykiss
- Source: Aquatic Research organisms, Hampton, NH
- Age at study initiation: 64 days (Hatch: 11/09/90)
- Length at study initiation: ≤50 mm total length, uniform size
- Feeding during test: None
ACCLIMATION:Test fish were acclimated to the receiving water for a minimum of 24 hours - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- None
- Hardness:
- 80-100 mg/L as CaC03.
- Test temperature:
- 15°C±2°C
- pH:
- 7.4 to 7.8.
- Dissolved oxygen:
- 7.4-8.6
- Nominal and measured concentrations:
- Nominal : 0, 62.5, 125, 250, 500, 1000 ppm
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 4L polypropylene vessels; exposure volume 3000 ml
- Aeration: free air supplied at ≤100 bubbles per minute to maintain ≥ 60% saturation.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per vehicle control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: EPA moderately hard reconstituted (from EPA 600/4-85/013):
Reconstituted freshwater
NaHCO3 = 96 mg/L
CaSO4.2H20 = 60 mg/L
MgSO4 = 60 mg/L
KCI = 4 mg/L
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16hr/8hr light/dark cycle
- Light intensity: fluorescent, 50 to 100 foot-candles (lab ambient)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, behaviour (lethargy, erratic swimming), and physiology (discoloration), initially, and daily thereafter. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 500 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: not obtainable graphical interpolation
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 360 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: not obtainable graphical interpolation
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 240 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: not obtainable graphical interpolation
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 180 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: not obtainable graphical interpolation
- Sublethal observations / clinical signs:
Acute Toxicity versus Onchorhyncus mykiss Definitive Test - CT 444 90 D
Conc.( ppm) Number Organism Cumulative Mortality 24 h 48 h 72 h 96 h % Mortality 0 control 10 0 0 0 0 0 62.5 10 0 0 0 1 10 125 10 0 0 0 0 0 250 10 0 0 6 10 100 500 10 5 10 10 10 100 1000 10 10 10 10 10 100 - Validity criteria fulfilled:
- yes
- Remarks:
- all of control organisms survived 96 hours
- Conclusions:
- Acute toxicity of acrylamide (CT 444 90 D) to the Rainbow Trout, Onchorhyncus mykiss, was determined to be 180 ppm.
Reference
Description of key information
The acute toxicity of the read-across material, acrylamide, to fish was assessed according to OECD Test Guideline 203. The 96 h LC50 was 180 ppm.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 180 mg/L
Additional information
The short term toxicity to fish of the read-across material, acrylamide, was assessed according to OECD Test Guideline 203. Freshwater trout (Oncorhynchus mykiss) were exposed to the read-across material for 96 hours in a static freshwater test at nominal concentrations of 0, 62.5, 125, 250, 500 and 1000 ppm in open 4 L polypropylene vessels. Mortality, behaviour (lethargy, erratic swimming), and physiology (discoloration) were measured initially and daily thereafter.
At 250, 500 and 1000 ppm there was 100 % mortality. No mortality was observed at 125 ppm, and 10 % mortality at 62.5 ppm. The LC50 value was based on graphical interpolation.
The 96 hour LC50 was 180 ppm.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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