Residues, zinc smelting

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

not applicable

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Not according to GLP nor to specific test guidelines. Only 1 bacterial strain is used.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Principles of method if other than guideline:

Bacterial gene mutation assay was used to test the test material for reversion of his- auxotrophs of Salmonella Thyphimurium in the strains TA1535, TA1537, TA98 and TA100 and tryp of Escherichia Coli wp2 uvrA

GLP compliance:

not specified

Type of assay:

bacterial gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Residues, zinc smelting

EC Number:

273-824-3

EC Name:

Residues, zinc smelting

Cas Number:

69029-83-0

Molecular formula:

Not available, UVCB substance.

IUPAC Name:

Residues, zinc melting, alloying and casting

Details on test material:

name of test substance: Cenizas de cinc de segunda fusion
origin of test substance: Befesa Zinc Amorebieta

Method

Target gene:

not applicable

Species / strain

Species / strain / cell type:

E. coli WP2 uvr A

Details on mammalian cell type (if applicable):

not applicable

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

not specified

Test concentrations with justification for top dose:

312; 625; 1250; 2500 and 5000 µg/plate

Vehicle / solvent:

phosphate buffer

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Remarks:

benzoapyrene

Positive control substance:

no

Remarks:

none

Details on test system and experimental conditions:

plate method: plate incorporation
endpoint: gene mutation

Evaluation criteria:

no information

Statistics:

no statistics reported

Results and discussion

Test results

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Remarks:

in vitro gene mutation

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

none

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

none

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

This study reported that the test material was negative in Escherichia Coli with and without metabolic activation tested from 312-5000 µg/plate

Executive summary:

This study report demonstrated no mutagenic effect for the sample 'Cenizas de cinc de segunda fusion'