2-chloroethanol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05-1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non GLP no reference substance and no analysis. However the test is well documented and the guideline was valid at that time. The data provided and the study design is enough to consider K2 as reliability.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Algen-Wachstumshemmtest DIN 38412/9

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-chloroethanol

EC Number:

203-459-7

EC Name:

2-chloroethanol

Cas Number:

107-07-3

Molecular formula:

C2H5ClO

IUPAC Name:

2-chloroethan-1-ol

Details on test material:

2-chloroethanol

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Substance is a colorless liquid and is very soluble in water, boilingpoint is 129 C and the pH <7, the substance is stable under ambient conditions

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

cultivated at pH 8.2+-0.3 in 250 ml lage Erlemeyer with 100ml medium

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

20

pH:

8.3-9.2

Nominal and measured concentrations:

nominal: 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 mg/L

Details on test conditions:

start with 10000 cells per ml. the test solution was transfered to 10ml bekers with as much solution. Illumination was at 120uE/m2s directed from above

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.95 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

5.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

30.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Reported statistics and error estimates:

mono variable linear regression and graphical analysis

Any other information on results incl. tables

Concentration mg/L	Biomass	growthrate
0	1.6	1.16
0.20	1.54	1.12
0.40	1.43	1.12
0.80	1.20	1.06
1.60	0.94	0.97
3.20	0.67	0.70
6.40	0.63	0.57

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Study is valid however concentration is based on nominal. no reference substance is used so the control cannot be done. Therefore the use of this study is limited when applying it in a riskassessment

Executive summary:

An algal essay was performed according to the DIN 38412/9 Guideline with 2 -chloroethanol. The study design was static with 72h exposure. No reference substance was used. pH varied between 8.3 -9.2 and this range is acceptable especially in an algal essay. the EC10 for growth rate is to be sued when applying the result in a riskassessment and was determined via statistical extrapolation to be 0.95 mg/L of nominal test substance concentration.