DL-2-aminopropan-1-ol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

effects on growth of green algae

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

1

Reference substance name:

DL-2-aminopropan-1-ol

EC Number:

228-207-3

EC Name:

DL-2-aminopropan-1-ol

Cas Number:

6168-72-5

Molecular formula:

C3H9NO

IUPAC Name:

2-aminopropan-1-ol

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

measured at 0 and 72h

Test solutions

Vehicle:

no

Details on test solutions:

0 (control), 0.095, 0.31, 1.0, 3.1, and 10 mg 2-Aminopropanol/L

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

22.6-24.7

pH:

7.5-9.3 (test item-dependent increased pH at t0)

Nominal and measured concentrations:

Measured concentrations at initiation and termination were respectively within 100-106% and 94-103% of the nominal concentrations. The biological response is thus reported based on initial measured concentrations.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.18 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: calculated from raw data

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.099 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: this is considered less relevant for classification than the ErC10, which better reflects the dose-response-curve

Effect concentrations 3

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

7.85 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

achieved concentrations were stable over 72h

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

In a static test, algae ErC50(72h) was 7.85 mg/L, NOEC(growth rate) was 0.0991 mg/L, and ErC10 was 0.18 mg/L based on measured initial concentrations of DL-2AP. Measured concentrations were stable over 72h.