bis(trifluoromethylsulfonyl)azanide;1-ethyl-3-methylimidazol-3-ium

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study Plan dated: 24.Sep.2018; Experimantal Starting Date: 19.Mar.2019; Experimental Completion Date: 21.Mar.2019

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

13. April 2004

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

30. May 2008

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: OECD guidance document No.23, GUIDANCE DOCUMENT ON AQUATIC TOXICITY TESTING OF DIFFICULT SUBSTANCES AND MIXTURES

Version / remarks:

06.Jul. 2018

GLP compliance:

yes (incl. QA statement)

Remarks:

Landesamt für Umwelt; Kaiser-Friedrich-Straße 7, 55116 Mainz

Test material

Test material information

Constituent 1

Reference substance name:

1-ethyl-3-methyl-1H-imidazol-3-ium; trifluoro[(trifluoromethanesulfonylazanidyl)sulfonyl]methane

EC Number:

700-235-5

Cas Number:

174899-82-2

Molecular formula:

C8H11F6N3O4S2

IUPAC Name:

1-ethyl-3-methyl-1H-imidazol-3-ium; trifluoro[(trifluoromethanesulfonylazanidyl)sulfonyl]methane

Test material form:

liquid

Details on test material:

clear liquid, colorless

Specific details on test material used for the study:

- Source and lot/batch No.of test material: proionic GmbH, 25PI208_12
- Expiration date of the lot/batch: 01.05.2019
- Purity test date: EMIM+: 99.84.wt%; NTF2-: 99.95 wt%

- Storage condition of test material: room temperatur, in proper conditions

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Species Daphnia magna
Authority STRAUS
Strain Berlin
Sex female
Age between 0 and 24 hours
Origin Umweltbundesamt Berlin
In-house breeding since 27. September 2007

Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identi-cal. The keeping is performed similar to the method described in the OECD guideline, fol-lowing SOP 115 002 01 („Zucht und Hälterung von Daphnia magna STRAUS“), version 12 from 02. Feb. 2015.
Vessels preserving glasses, nominal volume 2 L
Medium M4-Medium (recipe of ELENDT)
Food green algae (Desmodesmus subspicatus)
Medium renewal twice a week
Photo period 16/8 hours, using neon tubes
Temperature 20  2 °C

Study design

Water media type:

other: dilution water

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

20.8 - 22.0 °C

pH:

Nominal Concentration in mg/L pH
0 h 48 h
Blank control 7.8 7.7
100 7.8 7.8

Dissolved oxygen:

Nominal Concentration in mg/L O2-Concentration in mg/L
0 h 48 h
Blank control 8.8 8.9
100 8.8 8.7

Nominal and measured concentrations:

Nominal
Concentration Calculated Concentration
Test Item test item in mg/L % of Nominal
mg/L t = 0 h t = 48 h mg/L mg/L
Blank control < LOQ < LOQ -- --
100 98.06 109.03 98 109

Details on test conditions:

Date of performance 19. – 21. March 2019
Treatments 100 mg/L (nominal concentration)
The concentrations to be tested are based on the result of a non-GLP pre-test.
Temperature 20.8 – 22.0 °C
Duration 48 hours
Observation times 24 and 48 hours
Medium renewal none
Test vessels glass beakers, nominal volume 50 mL, tall shape
Replicates (Treatments) 4 vessels, each containing 20 ± 5 mL test solution and 5 daphnia
Replicates (Blank control) 4 vessels, each containing 20 ± 5 mL dilution water and 5 daphnia

Reference substance (positive control):

yes

Remarks:

Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 110.1 mg/L

Nominal / measured:

nominal

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 110.1 mg/L

Nominal / measured:

nominal

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 110.1 mg/L

Nominal / measured:

nominal

Effect concentrations 4

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

>= 110.1 mg/L

Nominal / measured:

nominal

Any other information on results incl. tables

None of the animals was immobilised in the blank control and the test solution. Therefore no statistical evaluation was performed

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Immobilisation in the controls may not exceed 10% .Immobilisation in the controls was 0%.The concentration of dissolved oxygen at the end of the test must be at least 3mg/L.The lowest concentration of dissolved oxygen at the end of the test was 8.7mg/L.

Executive summary:

One valid experiment was performed.

The study was performed as a limit test at 100 mg/L (nominal concentration). For each test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.

None of the animals was immobilised in the blank control and the test solution. Therefore no statistical evaluation was performed.

 

Potassium dichromate K₂Cr₂O₇(CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable.

 

At the beginning and at the end of the test, the content of the test item in the test solutions was determined using LC-MS-MS-determination. The concentration determined at the start of the test was 98 % of the nominal concentration. At the end of the test the determined concentration was 109 % of the nominal concentration. Therefore, the determination of the biological results was based on the nominal concentration (cf. Guidance Doc. No. 23 §176).

The following results were determined for the test item1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (species: Daphnia magna).

 

48h-NOEC≥100 mg/L (nominal conc.)
48h-LOEC> 100mg/L (nominal conc.)
24h-EC₅₀> 100 mg/L(nominal conc.)
48h-EC₅₀> 100 mg/L(nominal conc.)