Diisooctyl succinate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Diisooctyl succinate

EC Number:

249-282-9

EC Name:

Diisooctyl succinate

Cas Number:

28880-24-2

Molecular formula:

C20H38O4

IUPAC Name:

bis(6-methylheptyl) succinate

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Remarks:

Carboxymethyl Cellulose (at 30 mg/l concentration)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Name of Species : Daphnia magna
No. of Daphnia group : 20 Daphnids per concentration (four replicates of single
Concentration and each containing Five Daphnids)
Temperature : 18 - 22°C
No. of Dose concentration : 5

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

21.2-21.6 °C

pH:

6.0-7.2

Dissolved oxygen:

≥ 4 mg/l content of the water

Nominal and measured concentrations:

A limit test was performed at a dose concentration of 100 mg/l of the test
item prior to the main study. On the basis of the findings main study was
conducted by preparing five concentrations of the test item (i.e. 2, 4, 8,
10, 20 mg/l).

Details on test conditions:

Immobilization : In the control not more than 10 % of the Daphnia should immobilize during the test period.
Light conditions : 16 hours light and 8 hours dark cycle

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

9.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95 % F. limit : 7.16- 11.77

Details on results:

EC50 : 9.30 mg/l of water
95 % F. limit : 7.16- 11.77
Lowest concentration causing 100 % immobility in Daphnia : 40 mg/ l of water

Reported statistics and error estimates:

EC50 was calculated using Finney’s Probit Analysis

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this study toxicity to Daphnia i.e.EC50 of “Dicapryl Succinate” came out as:
EC50 : 9.30 mg/l of water
95 % F. limit : 7.16- 11.77
The compound has been tested as per ‘OECD Guideline for the testing of Chemicals, Daphnia Acute Immobilization Test Guideline No. 202 adopted on 13th April 2004’

Executive summary:

The study was conducted to determine the ‘Immobilization test of ‘Dicapryl Succinate’ on a benthic zooplankton strain ‘Daphnia magna’.

In the present study, a limit test was performed at 100 mg/l concentration, of the test item ‘Dicapryl Succinate’ with 20 Daphnia (four replicates of five Daphnids each). Based on the result of the limit test, the Main Test was conducted taking five different concentrations of the test item (20, 40, 60, 80 and 100 mg/l). Similarly 20 Daphnia (four replicates of five Daphnids each) for negative control (water) and solvent control (at 30mg/l concentration of CMC) groups were used (the volume of solvent, used in the test concentrations and control was kept same).

On the basis of these concentrations, the EC50 was estimated and the lowest concentration causing 100 % immobility, were determined, and which came out as:

EC50 : 9.30 mg/l of water

95 % F. limit : 7.16- 11.77

Lowest concentration causing 100 % immobility in Daphnia : 40 mg/ l of water