N,N-dimethylbenzamide

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

single dose application, counting deaths after 48 hours

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Data source

Reference

Reference Type:

review article or handbook

Title:

Unnamed

Year:

1971

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The compounds were reduced to fine powder, suspended in 1% methylcellulose mucilage and homogenized. The suspension was administered orally to groups of 20 mice using a dose volume of 0.5 ml/20g bw. Deaths were counted after 48h and the LD50 limits were calculated.
Analgesic properties of the compounds were detected by hotplate and writhing test. In the hotplate test, 20 mice were used at each of the 4 dose levels. Every 30 min for 3 hours the mice were placed at a hotplate (55 +/- 0.5 °C) for 20 sec. The flicking of the hind paws were counted. 20 mice given 1% methylcellulose only were used as control.
For the writhing test 12 mice were dosed orally with the test compound. 30 min. later formic acid (25 mg/kg bw, ip) was injected. The animals were placed individually in observation chambers and the number of writhing episodes within the next 20 min. was counted. A group of 12 mice, treated with methylcellulose only served as control.
ED50 (hotplate or writhing) were calculated by standard statistical methods.

GLP compliance:

no

Test type:

other:

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

N,N-dimethylbenzamide

EC Number:

210-279-2

EC Name:

N,N-dimethylbenzamide

Cas Number:

611-74-5

Molecular formula:

C9H11NO

IUPAC Name:

N,N-dimethylbenzamide

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

Amides were prepared by standard methods from the appropriate amines and acids, obtained commercially or prepared by published methods.

Test animals

Species:

mouse

Strain:

other: albinos from the QS strain

Sex:

female

Details on test animals or test system and environmental conditions:

groups of 20 female mice, weighing 18-22 g for LD50 test
4 groups of 20 female mice, weighting 18-22 g for the hotplate test, another group of 20 mice served as control
4 (?) groups of 12 female mice, weighting 18-22 g for the writhing test, another group of 12 mice served as control

Administration / exposure

Route of administration:

oral: unspecified

Details on oral exposure:

The compounds were reduced to fine powder, suspended in 1% methylcellulose mucilage and the mixture was homogenized. The suspension was administered orally to groups of mice using a dose volume of 0.5 ml/ 20 g bw.

Doses:

not specified in the article
1 dose level for the LD50 test
4 dose levels for the hotplate test
some dose levels for the writhing test

No. of animals per sex per dose:

20 females for LD50 test
20 femals per dose for the hotplate test + 20 female for the control
12 females per dose for the writhing test + 12 females for the control

Control animals:

other:

Remarks:

none for LD50, 20 for hotplate, 12 for writhing

Details on study design:

LD50: oral application in 20 mice. Deaths were counted after 48 hours
Analgesic properties: Hotplate test and writhing test

Statistics:

LD50, ED50 (hotplate) and ED50 (writhing) including confidence limits were calculated by the method of Litchfield and Wilcoxon (1949).

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 960 mg/kg bw

Based on:

not specified

Remarks on result:

other: 807-1142 mg/kg bw

Remarks:

limits of error for P = 0.05

Effect levels 2

Sex:

female

Dose descriptor:

other: ED50

Remarks:

hotplate

Effect level:

ca. 250 mg/kg bw

Based on:

not specified

Effect levels 3

Sex:

female

Dose descriptor:

other: ED50

Remarks:

writhing

Effect level:

ca. 211 mg/kg bw

Based on:

not specified

Mortality:

not specified

Clinical signs:

analgetic properties
depressant activities at high doses, ataxia, cyanosis of ears, tail and feet

Body weight:

18-22 g at the beginning of the test

Gross pathology:

not mentioned

Other findings:

analgesic properties

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

According to this publiction the test substance has a LD50 = 960 mg/kg bw for female mice. It also has analgesic properties.