Fatty acids, C16-18, 2-butyloctyl esters

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

expermimental period: January 19th - 21st 2005

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTIONS
- Method: stirring 100.2 mg/l of the test item in Daphnia dilution water according to OECD 202 for 24 hours followed by filtration with a 0.45 µm CA-filter (cellulose acetate syringe filter, Schleicher&Schuell PP 30/0.45 C); the water soluble fraction (WSF) was tested
- Controls: Daphnia dilution water according to OECD 202

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM:
- Common name: Daphnia magna Straus
- Source: German Federal Environment Agency,
the current breeding was held at Hydrotox GmbH since October 2001; Twice a week the animals are placed into fresh Elendt-Medium M4 (acc. OECD 202). They are kept at approximately 10 animals/200 ml; five days a week they are fed with a suspension of the green algae Desmodesmus subspicatus; During the test Dapnia were not fed.

ACCLIMATION
Daphnia are held at 20 +/- 1°C with a light dark cycle of 12:12 h; the breeding was stable since August 1999; the test was performed under the same conditions but in the dark

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

non

Hardness:

not determined

Test temperature:

20-21°C

pH:

control: 7.7 (0h) and 7.6 (48h)
100 mg/l: 7.7 (0h) and 7.7 (48h)

Dissolved oxygen:

control: 0h - 8.8 and 48h - 8.7 mg/L
100 mg/l: 0h - 8.8 and 48 h - 8.8 mg/L

Nominal and measured concentrations:

nominal concentration: 100.2 mg/l (nominal loading rate)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- glass beaker 50 ml filled with a test volume of 20 ml; no aeration
- no renewal of test solution during the test duration of 48 hours
- 5 Dapnia per vessel
- 4 replicate vessels per concentration and control

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water according to OECD 202;
- Culture medium different from test medium: yes, culture medium: Elendt-Medium M4 according to OECD 202
- Intervals of water quality measurement: conductivity of the deionised water used for the preparation of the culture medium and the dilution water is measured dayly

OTHER TEST CONDITIONS
- Adjustment of pH: pH of the test solution was not adjusted
- Photoperiod: the test was performed in the dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
After 24 and 48 hours the test vessels were taken out from the incubator and put onto a black cloth, and the swimming capability of the Daphnia was observed. An animal that did not swimm within 15 seconds after gently moving the beaker was considered to be immobile.

TEST CONCENTRATIONS
the test was performed as Limit test with a test concentration of 100.2 mg/l

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

24 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

mobility

Details on results:

No effect on the swimming capability (mobility) of Dapnia with the water soluble fraction (WSF). The EL50 > 100.2 mg/l. The test was performed without accompanying chemical analysis due to the insoluble nature of the test item.

Results with reference substance (positive control):

EC50 for the reference substance potassium dichromate determined in September 2004; results are not given in the report

Validity criteria fulfilled:

yes

Conclusions:

The study was performed as limit test with the water soluble fraction (WSF) after 24 hours stirring and filtration and a nominal test concentration of
100.2 mg/l. No effects on the swimming capability of the Daphnia could be observed. The EL50 (48 h) is > 100.2 mg/L

Executive summary:

The study was performed as limit test with the water soluble fraction (WSF) after 24 hours stirring and filtration and a nominal test concentration of 100.2 mg/l. No effects on the mobility of the Daphnia could be observed. The EC50 (48 h) is > 100.2 mg/L.

Description of key information

In a well conducted OECD 202 study with the target substance Fatty acids, C16-18, 2-butyloctyl esters the EL50 (48 h) is > 100 mg/L.  

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

An OECD 202 test was performed with the target substance Fatty acids, C16-18, 2-butyloctyl esters as limit test with the water soluble fraction (WSF) after 24 hours stirring and filtration and a nominal test concentration of 100.2 mg/l. No effects on the mobility of the Daphnia could be observed. The EL50 (48 h) is > 100.2 mg/L.