Sodium hydrogen 8-aminonaphthalene-1,6-disulphonate

Endpoint summary

Description of key information

Short term toxicity study to aquatic invertebrates:

Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on Daphnia magna was predicted for sodium hydrogen 8-aminonaphthalene-1,6-disulfonate (74543-22-9). The EC50 value was estimated to be 194.355 mg/l when sodium hydrogen 8-aminonaphthalene-1,6-disulfonateexposed to Daphnia magna for 48hrs.

Toxicity to aquatic algae and cyanobacteria:

Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the seven closest read across substances, toxicity on Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) was predicted for sodium hydrogen 8-aminonaphthalene- 1,6-disulfonate (74543-22-9). The EC50 value was estimated to be 199.6697 mg/l when sodium hydrogen 8-aminonaphthalene-1,6-disulfonate exposed to Pseudokirchneriella subcapitata for 72hrs. Thus the chemical was consider as nontoxic to aquatic algae.

Additional information

Summarized result of toxicity of sodium hydrogen 8-aminonaphthalene-1,6-disulfonate (74543-22-9) on the growth and other activity of aquatic invertebrates, algae and cyanobacteria by considering the data for target as well as RA chemical which was selected on the basis of structure similarity are as follows:

Short term toxicity study to aquatic invertebrates:

Based on the various experimental data and prediction data for the target chemical, study have been reviewed to determine the toxic nature of sodium hydrogen 8-aminonaphthalene-1,6-disulfonate (74543-22-9) on the growth of aquatic invertebrates. The studies are as mentioned below

In the first weight of evidence predicted study for sodium hydrogen 8-aminonaphthalene-1,6-disulfonate (74543-22-9) from QSAR toolbox, 2017 Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on Daphnia magna was predicted for sodium hydrogen 8-aminonaphthalene-1,6-disulfonate (74543-22-9). The EC50 value was estimated to be 194.355 mg/l when sodium hydrogen 8-aminonaphthalene-1,6-disulfonateexposed to Daphnia magna for 48hrs.

 

Similarly in another expiremental report for RA chemical 4-amino-5-hydroxyna phthalene-1,7- disulphonic acid (130-23-4) from ABITEC lab report, 2016, Determination of the inhibition of the mobility of daphnids was carried out with the substance 4-amino-5-hydroxyna phthalene-1,7- disulphonic acid according to OECD Guideline 202. The test substance was tested at the concentrations 0, 0, 0, 5, 10, 25, 50, 100, 200 mg/l.. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, 4-amino-5-hydroxyna phthalene-1,7- disulphonic acid in Daphnia magna was determined to be 500 mg/L for immobilisation effects. This value indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.

 

Similarly in the third weight of evidence study for the RA chemical (121-57-3) from chemosphere 1994, Toxicological profile of sulphanilic acid were study on the daphnia magna for 24 hrs. From the study the EC50 value for short term toxicity to aquatic invertebrates for sulphanilic acid in 24 hrs was determined to be 109.13 mg/l. Based on the result, sulphanilic acid (121-57-3) was considered as not toxic and not classified as per the CLP regulations.

 

Similarly in the fourth weight of evidence study for the RA chemical (121-57-3) from IUCLID dataset, The short term toxicity study of sulphanilic acid to aquatic invertebrates was performed on Daphnia magna. Test was performed by static system. After the 24 hrs of exposure with chemical, the EC50 and EC100 value for Daphnia magna for Sulphanilic Acid (121-57-3) was determined to be 109.13 and 250 mg/l. Based on the value the sulphanilic Acid was considered to be non-toxic to aquatic invertebrate and can be considered to be not classified as per CLP regulations.

 

Based on the data obtain from various sources for target and RA chemicals, it can be concluded that the substance sodium hydrogen 8-aminonaphthalene-1,6- disulfonate (74543-22-9) is considered to be not toxic to aquatic environment and cannot be classified as toxic as per the criteria mentioned in CLP regulation.

Toxicity to aquatic algae and cyanobacteria:

Based on the various experimental data and prediction data for the target chemical, study have been reviewed to determine the toxic nature of sodium hydrogen 8-aminonaphthalene-1,6-disulfonate (74543-22-9) on the growth of aquatic algae and cyanobacteria. The studies are as mentioned below

 

In the first weight of evidence predicted study for sodium hydrogen 8-aminonaphthalene-1,6-disulfonate (74543-22-9) from QSAR toolbox, 2017. Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the seven closest read across substances, toxicity on Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) was predicted for sodium hydrogen 8-aminonaphthalene- 1,6-disulfonate (74543-22-9). The EC50 value was estimated to be 199.6697 mg/l when sodium hydrogen 8-aminonaphthalene-1,6-disulfonate exposed to Pseudokirchneriella subcapitata for 72hrs. Thus the chemical was consider as nontoxic to aquatic algae.

 

Similarly in another weight of evidence study report for RA chemical 4-amino-5- hydroxy naphtha- lene-1,7-disulphonic acid (130-23-4) from ABITEC report, 2017. Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 4-amino-5- hydroxy naphtha- lene-1,7-disulphonic acid according to OECD Guideline 201. The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 2.2, 11, 25, 55 and 120 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (ErC50) for the test substance, 4-amino-5-hydroxynaphthalene -1,7- disulphonic acid, in Desmodesmus subspicatus was determined to be 129.4 mg/L. Based on this ErC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance 4-amino-5-hydroxynaphthalene-1,7-disulphonic acid does not exhibit toxicity to aquatic algae (Desmodesmus subspicatus) and cannot be classified as toxic as per the CLP classification criteria.

 

Similarly in another weight of evidence study report for RA chemical 4-amino-5- hydroxy naphtha- lene-1,7-disulphonic acid (130-23-4) from UERL report, 2017. The effect of test item disodium 4-amino-5-hydroxy naphtha -lene-1,7-disulphonic acid, was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25mg/l, 12.5mg/l, 25mg/l, 50mg/l, 100mg/l and 200mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be >200mg/l. Thus, based on the EC50 value, test substance can be classified as not hazardous as per the criteria of CLP regulation.

 

In the fourth weight of evidence study for the RA chemical 4-amino-3- methylbenzene sulfonic acid (98-33-9) from ABITEC report, 2017.Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 4-amino-3- methylbenzene sulfonic acid according to OECD Guideline 201. The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 0, 30, 45, 67, 100, 150 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance 4-amino-3-methylbenzene- sulfonic acid, in Desmodesmus subspicatus was determined to be 110.6 mg/L. This value indicates that the substance is likely to be non-hazardous to aquatic algae and cannot be classified as per the CLP criteria.

 

Based on the data obtain from various sources for target and RA chemicals, on the toxicity to invertebrates and algae, it can be concluded that the substance sodium hydrogen 8-aminonaphthalene-1,6- disulfonate (74543-22-9) is considered to be not toxic to aquatic environment and cannot be classified as toxic as per the criteria mentioned in CLP regulation.