Registration Dossier
Registration Dossier
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EC number: 221-717-7 | CAS number: 3209-22-1
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well-documented study report which meets basic scientific principles.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 983
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- tester strains used not in line with current guideline
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,2-dichloro-3-nitrobenzene
- EC Number:
- 221-717-7
- EC Name:
- 1,2-dichloro-3-nitrobenzene
- Cas Number:
- 3209-22-1
- Molecular formula:
- C6H3Cl2NO2
- IUPAC Name:
- 1,2-dichloro-3-nitrobenzene
- Details on test material:
- 2,3-dichloronitrobenzene
Constituent 1
Method
- Target gene:
- Ames test: single-base mutations, frame-shift mutations
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: tester strains: TA100, TA1535, TA1537, TA98
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix (S9 homogenate from induced male Sprague Dawley rat liver S) and from induced male Syrian hamster liver S9)
- Test concentrations with justification for top dose:
- 0, 33, 100, 1000, 333 and 10000 µg/plate
- Vehicle / solvent:
- dimethyl sulfoxide
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: -S9: sodium azide (TA 100, TA 1535), 2-nitrofluorene or 4-nitro-o-phenylenediamine (TA 98), 9-aminoacridine (TA1537); +S9: 2-aminoanthracene (all strains)
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: tester strains: TA100, TA1535, TA1537, TA98
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- tester strain TA100 (with metabolic activation)
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- 2,3-dichloronitrobenzene induced a weak positive response in the ames assay in presence of metabolic activation.
- Remarks on result:
- other: strain/cell type: S. typhimurium tester strains: TA100, TA1535, TA1537, TA98
Applicant's summary and conclusion
- Conclusions:
- 2,3 -Dichloronitrobenzene induced a weak mutagenic response in bacteria.
- Executive summary:
The mutagenic potential of 2,3 -Dichloronitrobenzene was evaluated in the Ames test (NTP 1981). The test substance induced a weak positive response in Salmonella typhimurium tester strain TA100 in presence of metabolic activation.
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