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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2020-02 to 2020-05
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
mixed population of aquatic microorganisms (activated sludge)
Origin : aeration tank of a wastewater plant treating predominantly domestic sewage (Wupper area water authority, WWTP Odenthal)
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
The endogenous activity of the inoculum was checked running parallel blanks with inoculum but without test item. A reference compound (sodium benzoate) was run in parallel to check the operation of the procedures.
A toxicity control (test item and reference compound mixed, one replicate) was run in parallel, to ensure that the chosen concentration of the test item was not inhibitory to microorganisms.
Reference substance:
benzoic acid, sodium salt
Key result
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
7 d
Key result
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
14 d
Key result
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
21 d
Key result
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d
Parameter:
COD
Value:
1.176 mg O2/g test mat.
Parameter:
ThOD
Value:
1.665 mg O2/g test mat.
Results with reference substance:
87 % degradation with sodium benzoate was achieved.
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
4-trifluoromethoxyaniline is not considered to be readily biodegradable, as the substance showed 0 % degradation (COD) after 28 days.
Executive summary:

The biodegradation of the test item was 0 % (COD) after 28 days. The test item failed the pass level requested in test method OECD 301 F which is a biodegradation ≥ 70 % DOC or ≥ 60 % COD.

The test item is, therefore, considered to be not readily biodegradable.

The reference compound sodium benzoate showed 87 % degradation after 14 days.

Description of key information

The biodegradation of the test item was 0 % (COD) after 28 days. The test item failed the pass level requested in test method OECD 301 F which is a biodegradation ≥ 70 % DOC or ≥ 60 % COD.

The test item is, therefore, considered to be not readily biodegradable.

The reference compound sodium benzoate showed 87 % degradation after 14 days.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:
freshwater

Additional information