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EC number: 241-304-5 | CAS number: 17268-47-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-09-19 to 2018-11-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK.
- Method of cultivation: The activated sewage sludge sample was washed twice by settlement and re-suspension in mineral medium to remove any excessive amounts of Dissolved Organic Carbon (DOC) that may have been present.
- Storage conditions: The washed sample was maintained on continuous aeration in the laboratory at a temperature of approximately 21 ºC and used on the day of collection.
- Pretreatment: none
- Concentration of sludge: 30 mg dry solids per litre
- Initial cell/biomass concentration: The suspended solids concentration was 2.1 g/L prior to use. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 10 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
A: Potassium dihydrogenphosphate KH2PO4 8.50 g
Dipotassium hydrogenphosphate K2HPO4 21.75 g
Disodium hydrogenphosphate dihydrate Na2HPO4 * 2 H2O 33.40 g
Ammonium chloride NH4Cl 0.50 g
are dissolved in demineralised water and made up to 1 litre.
B: Calcium chloride CaCl2 27.50 g
is dissolved in demineralised water and made up to 1 litre.
C: Magnesium sulfate heptahydrate MgSO4 * 7H2O 22.5 g
is dissolved in demineralised water and made up to 1 litre.
D: Iron (III) chloride hexahydrate FeCl3 * 6H2O 0.25 g
is dissolved in demineralised water, stabilised with one drop of concentrated HCl and made up to 1 litre.
For preparation of the mineral medium 10 mL of solution (A) is mixed with 900 mL demineralised water, 1 mL each of solutions (B), (C) and (D) are added and the volume is made up to 1 litre.
- Additional substrate:
- Solubilising agent (type and concentration if used):
- Test temperature: 22 °C - 24 °C
- pH: 7.4 (day 0 post-adjustment) to 7.5 (day 28)
- pH adjusted: yes
- Suspended solids concentration: 2.1 g/L
- Continuous darkness: yes
- Other: dry solid concentration: 180 mL activated sludge was filled up to 3000 mL with 2820 mL mineral medium corresponding to 30 mg/L dry solids.
TEST SYSTEM
- Culturing apparatus: not stated
- Number of culture flasks/concentration: 2 vessels in the inoculated control, the procedure control plus the reference item and the test item. 1 vessel only for the test item plus the reference item
- Method used to create aerobic conditions: The test vessels are aerated by the passage of carbon dioxide-free air.
- Measuring equipment: IC measurement was performed with a total carbon analyser (either TOC-V or TOC-L Shimadzu) by conversion of an aqueous sample to CO2 by orthophosphoric acid or 2M HCl using zero grade air as the carrier gas.
- Test performed in closed vessels due to significant volatility of test substance:
- Details of trap for CO2 and volatile organics if used: The CO2 produced by degradation was absorbed in two 500 mL Dreschel bottles each filled with 350 mL 0.05 M NaOH.
SAMPLING
- Sampling frequency: IC analysis samples on Days 0, 2, 6, 8, 10, 14, 21, 28 and 29 from the first CO2 absorver and days 0 and 29 for the second CO2 absorver.
- Sampling method: IC analysis: 2 mL samples from the first of two CO2-absorber flasks was sampled and the IC's were determined. On Day 28, 1 mL of concentrated hydrochloric acid was added to each vessel to drive off any inorganic carbonates formed. IC/TC: 30 mL samples from the inoculum control and test item vessels and filtered through 0.45 µm Gelman AcroCap fillters and the IC/TC were determined. Samples (50 µL) were injected into the TC and IC channels of the TOC analyzer.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- The results obtained from the samples taken for organic carbon analysis from the preliminary investigational work indicated that the test item did not adsorb to filter matrices or to activated sewage sludge. Therefore, for the purpose of the study, the samples taken for DOC analysis could be filtered to remove the suspended solids present without the loss of any test item.
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- - The degradation extent of the test substance at the end of the test was 0%.
- The degradation extent in the toxicity control was 30% within 14 days and 38% biodegradation after 28 days. The test substance had no inhibitory effect on the inoculum.
- The mean CO2-evolution of the blank flasks was 34.27 mg/L on day 28.
- Test control Sodium benzoate attained 70% biodegradation after 14 days with greater than 60% degradation being attained in a 10-Day window. After 28 days 87% biodegradation was attained. - Results with reference substance:
- The reference compound sodium benzoate reached the pass levels for ready biodegradability (61% ThCO2 by day 8) within 2 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The biodegradation of the test item was determined according to OECD 301 B under GLP conditions. The test item attained 0% biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No.301B.
- Executive summary:
The test item attained 0% biodegradation after 28 days. Under the strict terms and conditions of OECD Guideline No. 301B the test item cannot be considered to be readily biodegradable
Reference
Table 1 Inorganic Carbon Values on Each Analysis Occasion
Day | Inorganic Carbon (mg IC) | |||||||||||||
Inoculum Control | Procedure Control | Test Item | Toxicity Control | |||||||||||
R1 | R2 | R1 | R2 | R1 | R2 | R1 | ||||||||
Abs1 | Abs2 | Abs1 | Abs2 | Abs1 | Abs2 | Abs1 | Abs2 | Abs1 | Abs2 | Abs1 | Abs2 | Abs1 | Abs2 | |
0 | 1.75 | 1.75 | 1.75 | 1.75 | 1.75 | 1.05 | 1.75 | 1.17 | 1.75 | 1.75 | 1.75 | 1.05 | 1.75 | 1.75 |
2 | 5.45 | - | 6.61 | - |
19.72 |
- |
15.08 |
- |
7.89 |
- |
6.85 |
- |
20.53 |
- |
6 |
14.65 |
- |
14.07 |
- |
31.03 |
- |
27.91 |
- |
14.07 |
- |
13.73 |
- |
37.71 |
- |
8 |
12.84 |
- |
14.91 |
- |
35.09 |
- |
29.01 |
- |
14.68 |
- |
15.02 |
- |
33.83 |
- |
10 |
16.42 |
- |
16.65 |
- |
37.62 |
- |
35.34 |
- |
17.67 |
- |
11.29 |
- |
33.29 |
- |
14 |
19.72 |
- |
20.63 |
- |
44.88 |
- |
37.29 |
- |
19.04 |
- |
18.47 |
- |
38.31 |
- |
21 |
23.77 |
- |
28.28 |
- |
51.26 |
- |
45.40 |
- |
26.03 |
- |
26.59 |
- |
43.15 |
- |
28 |
37.47/26.32* |
- |
37.30/29.79* |
- |
61.93/53.76* |
- |
58.91/50.51* |
- |
41.22/27.55* |
- |
34.27/28.00* |
- |
63.28/49.73* |
- |
29 |
29.62 |
2.09 |
31.95 |
2.09 |
57.00 |
2.09 |
56.89 |
2.09 |
32.06 |
2.09 |
28.06 |
2.09 |
53.76 |
2.09 |
R = Replicate Abs = CO2 absorber vessel - = No value determined * Results from re-analysis of sample as original result was deemed to be erroneous
Table 2: Percentage Biodegradation Values
Day |
Biodegradation (%) |
||
Procedure Control |
Test Item |
Toxicity Control |
|
0 |
0 |
0 |
0 |
2 |
38 |
4 |
24 |
6 |
50 |
0 |
39 |
8 |
61 |
3 |
33 |
10 |
66 |
0 |
28 |
14 |
70 |
0 |
30 |
21 |
74 |
1 |
29 |
28 | 80 | 0 | 36 |
29* | 87 | 0 | 38 |
* Day 29 values corrected to include any carry-over of CO2 detected in Absorber 2
Description of key information
The test item attained 0% biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301B.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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