Registration Dossier
Registration Dossier
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EC number: 206-337-1 | CAS number: 328-84-7
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EPA-660/3-75-009
- GLP compliance:
- no
Test material
- Test material form:
- liquid
- Details on test material:
- Clear colourless liquid
- Specific details on test material used for the study:
- Purity: not reported
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Source: Union Carbide Environmental Services, Tarrytown, NY (original population of laboratory stock culture obtained from the EPA Environmental Research Laboratory, Duluth, Minnesota US)
- Age: less than 20 hours old
- Feeding during test: 1 ml prepared suspension of 10 g trout mash, 3 g Cerophyl, in 500 ml well water twice daily, 1.5 ml once daily on weekends.
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- 220 mg/L
- Test temperature:
- 20 +/- 3 °C
- pH:
- 7.71 to 8.01
- Dissolved oxygen:
- 8.0 mg/L
- Nominal and measured concentrations:
- Measured: 0.03, 0.06, 0.12, 0.24 and 0.38 mg/L
- Details on test conditions:
- - Renewal of test solution: Approximately every third day
- Photoperiod: 16 hours light; 8 hours dark.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 4 d
- Dose descriptor:
- LC50
- Effect conc.:
- >= 0.27 - <= 0.42 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 8 d
- Dose descriptor:
- LC50
- Effect conc.:
- >= 0.21 - < 0.33 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 15 d
- Dose descriptor:
- LC50
- Effect conc.:
- >= 0.17 - <= 0.31 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- >= 0.16 - <= 0.21 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of this test, the LC50 of 3,4-Dichlorobenzotrifluoride was reported to be 0.16 mg/L for day 21. 95% confidence limit: 0.12 - 0.21 mg/L.
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