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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
fish early-life stage toxicity
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EPA OTS 797.1000 (Fish Early-life Stage Toxicity Test)
GLP compliance:
yes

Test material

Test material form:
liquid
Details on test material:
Clear colourless liquid
Specific details on test material used for the study:
Purity: 99.5%

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Weekly sampling, starting at day 0.

Test solutions

Vehicle:
yes
Remarks:
Acetone

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Source: Mount Lassen Trout Farm, California
- Food type: live brine shrimp for larvae
- Amount: 3 times per on weekdays, 2 times per day on holidays and weekends

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
89 d
Remarks on exposure duration:
60 days post-hatch

Test conditions

Hardness:
22 to 32 mg/L
Test temperature:
10 to 14°C
pH:
6.9 to 7.4
Nominal and measured concentrations:
0.034, 0.068, 0.13, 0.25, 0.51 mg/L
Details on test conditions:
- Test solution: 90% replacement time ca. 8 to 9 h
- Photoperiod: 16 hours light and 8 hours darkness
- Fish per replicate: 20 larvae

Results and discussion

Effect concentrationsopen allclose all
Duration:
89 d
Dose descriptor:
NOEC
Effect conc.:
0.13 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Duration:
89 d
Dose descriptor:
LOEC
Effect conc.:
0.25 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Conclusions:
Under the conditions of this test for 3,4-Dichlorobenzotrifluoride the NOEC has been found to be 0.13 mg/L (89d) and the LOEC to be ca. 0.25 mg/L.