Ethyl (2E,4Z)-2,4-decadienoate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: FHSA, 16 CFR 1500.3(c)(2)(i)

GLP compliance:

yes

Test type:

fixed dose procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl (2E,4Z)-2,4-decadienoate

EC Number:

221-178-8

EC Name:

Ethyl (2E,4Z)-2,4-decadienoate

Cas Number:

3025-30-7

Molecular formula:

C12H20O2

IUPAC Name:

ethyl deca-2,4-dienoate

Test material form:

not specified

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature and humidity

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Ace Animals
- Weight at study initiation: 249-291 g
- Fasting period before study: 16-20 hours before dosing
- Housing: 5 per cage, suspended wire mesh cages
- Diet (e.g. ad libitum): Purina Rat Chow ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: at least one week

ENVIRONMENTAL CONDITIONS
- Temperature (°C): controlled
- Humidity (%): controlled
- Photoperiod (hrs dark / hrs light): 12 hrs light/12 hrs dark

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 5.0 g/kg

CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: If less than 5 rats at the 5.0 g/kg dose level die, then no further testing is needed.

Doses:

5.0 g/kg

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: 3-4 hrs after exposure, and daily thereafter
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, mortality

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

There was no mortalilty during the study.

Clinical signs:

other: See table below

Gross pathology:

Generally normal

Other findings:

- Histopathology: Animal #4 had hydronephrosis of the right kidney

Any other information on results incl. tables

Clinical Signs

Physical Sign	Maximum Number of Animals showing Sign at One Observation Period
Chromorhinorrhea	4
Ptosis	3
Chromodacryorrhea	2
Lethargy	1
Yellow Nasal Discharge	1
Anogenital Area Stained Brown	2

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The oral LD50 is > 5 g/kg in rats.

Executive summary:

The oral toxicity of ethyl decadienoate was tested in 10 rats. The 10 male rats were given doses of 5 g/kg of the test substance. They were then monitored for 14 days. No animals died during the study. Some minor clinical signs were noted, and only one abnormality was noted during the necropsies. The acute oral LD50 is therefore > 5 g/kg.