Ethyl (2E,4Z)-2,4-decadienoate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jan. 31-Feb. 12, 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl (2E,4Z)-2,4-decadienoate

EC Number:

221-178-8

EC Name:

Ethyl (2E,4Z)-2,4-decadienoate

Cas Number:

3025-30-7

Molecular formula:

C12H20O2

IUPAC Name:

ethyl deca-2,4-dienoate

Test material form:

not specified

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 2017284-0046
- Expiration date of the lot/batch: May 27, 2020

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

- Concentrations: All
- Sampling method: Samples were collected mid-depth. Samples were taken on Day 0 and at 24, and 48 hrs, except for the 3.2 mg a.il/L group which was not sampled after 24 hrs due to immobility.
- Sample storage conditions before analysis: Glass vials containing 5 mL of 0.2% phosphoric acid in methanol.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A 2000 mL stock solution was made at a concentration of 3.2 mg a.i./L, and sonicated for 60 min. It was then brought to final volume and stirred for 60 min. Aliquots of the stock solution were then diluted to produce the final test solutions, which were stirred for 15 min.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): None

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Justification for species other than prescribed by test guideline:
- Source: EAG Laboratories cultures, Easton, Maryland 21601
- Age of parental stock (mean and range, SD): at least 21 days
- Feeding during test: No

ACCLIMATION
- Health during acclimation (any mortality observed): mortality of the culture stock was <10% in the two days prior to the study

Study design

Test type:

other: static with renewal at 24 hrs

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

132 mg CaCO3/L

Test temperature:

20.1-21.0 degrees C

pH:

8.4-8.6

Dissolved oxygen:

>=90 ASV

Conductivity:

355 uS/cm

Nominal and measured concentrations:

Nominal Mean Measured
Negative Control < LOQ
0.20 mg a.i./L 0.18 mg a.i./L
0.40 mg a.i./L 0.34 mg a.i./L
0.80 mg a.i./L 0.66 mg a.i./L
1.6 mg a.i./L 1.5 mg a.i./L
3.2 mg a.i./L 2.6 mg a.i./L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed, covered loosely
- Material, size, headspace, fill volume: glass, 250 mL, 220mL
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): renewed at 24 hrs
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 40 m deep well in Easton, Maryland, USA, filtered and aerated, and passed through a UV sterilizer
- Alkalinity: 182 mg/L CaCO3
- Culture medium different from test medium: same


OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light/8 hrs dark with 30 minute transition periods
- Light intensity: 913 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobility

RANGE-FINDING STUDY
- Test concentrations: 1.2, 3.7, 11, 33 and 100 mg a.i./L
- Results used to determine the conditions for the definitive study: percent immobility was 50, 100, 100, 100 and 100% respectively in the range-finding study

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.4 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.66 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: 60% immobility was observed in the 1.5 mg a.i./L group, and 100% immobiilty in the 2.6 mg a.i./L group. Lethargy and floating was observed in the 0.66 mg a.i./L and 1.5 mg a.i./L group.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None

Reported statistics and error estimates:

95% Confidence Interval: 0.66 – 2.6 mg a.i./L

Any other information on results incl. tables

Concentration (mg a.i./L)	Number Immobilized (3 hrs)	Number Immobilized (24 hrs)	Number Immobilized (48 hrs)
0	0	0	0
0.18	0	0	0
0.34	0	0	0
0.66	0	0	0
1.5	0	0	12
2.6	0	20	20

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-hr EC50 for aquatic invertebrates was 1.4 mg a.i./L.

Executive summary:

The 48 -hr EC50 for aquatic invertebrates was tested in an OECD Guideline 202 study. Groups of Daphnia magna were exposed to concentrations of 0.18 mg a.i./L, 0.34 mg a.i./L, 0.66 mg a.i./L, 1.5 mg a.i./L, or 2.6 mg a.i./L (measured), for 48 hrs. 60% immobility was seen in the 1.5 mg a.i./L group, and 100% immobility in the 2.6 mg a.i./L group. No immobility was seen in other groups. The 48 -hr EC50 was therefore 1.4 mg a.i./L.