1,3,4-Thiadiazole-2(3H)-thione, 5-(tert-dodecyldithio)-

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1992-12-09 - 1992-12-10

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Study was conducted according to national guideline in Germany. No detailed information about GLP compliance available. This result is read-across from ‘1,3,4-Thiadiazolidine-2,5-dithione, reaction products with hydrogen peroxide and tert-dodecanethiol’. Read-across is justified as the two substances ‘1,3,4-Thiadiazolidine-2,5-dithione, reaction products with hydrogen peroxide and tert-dodecanethiol’ and ‘1,3,4-Thiadiazolidine-2,5-dithione, reaction products with hydrogen peroxide and tert-nonanethiol’ are virtually the same: the only difference between those two UVCB substances is that one of the used raw materials (alkanethiol) has a diversity in the C-range, i.e. on the one hand a tert. C12-alkanethiol is used in the manufacturing process, on the other hand a tert. C9. Hence, based on the (structural) similarity of both substances it is safe to say that the physicochemical, toxicological and ecotoxicological properties are likely to be similar.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,3,4-Thiadiazolidine-2,5-dithione, reaction products with hydrogen peroxide and tert-dodecanethiol

IUPAC Name:

1,3,4-Thiadiazolidine-2,5-dithione, reaction products with hydrogen peroxide and tert-dodecanethiol

Test material form:

liquid: viscous

Details on test material:

Not applicable.

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
- Name of test material (as cited in study report): Addition RC 8210
- Substance type: Dimercaptothiadizol-derivate
- Physical state: viscous, brownish liquid
- Smell: strong odor
- Water solubility: nonsoluble
- Storage condition of test material: room temperature, in the dark

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples were analyzed after the incubation period of 16 +/- 1 h.

Test solutions

Vehicle:

no

Details on test solutions:

- DILUTION WATER: deionized water

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Origin: Deutsche Sammlung für Mikroorganismen
- DMS number: 500 26
- Delivery date: 1992-09-18
- Age of the culture: the pre-culture is cultivated at 21 °C for 7 h before inoculation
- Pre-culturing: The preculture is diluted with a sterile nutrient solution until a turbity of 50 TE/F436 (turbidity of Formazine) is obtained.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

16 h

Remarks on exposure duration:

+/- 1 h

Post exposure observation period:

No post exposure observation period described.

Test conditions

Hardness:

No details available.

Test temperature:

21 +/- 1 °C

pH:

No details available.

Dissolved oxygen:

No details available.

Salinity:

Not applicable.

Nominal and measured concentrations:

80 and 8000 mg/L (8 g/L)

Details on test conditions:

TEST SYSTEM
300 mL Erlenmeyer flask on a shaker with incubation hood (Braun Certomat(R)).

TEST MEDIUM
An aqueous extract is prepared according to DEV S 4 using 100 mg test substance. This amount is mixed with 1 L deionized water and shaked overhead for 24 h. Filtration is done with the help of a rough fluted filter and the filtrate is used for testing.
In the second preparation, an eluate with 10 g/L test substance is used.

Reference substance (positive control):

no

Remarks:

No information about reference substance available.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

>= 8 g/L

Basis for effect:

growth inhibition

Remarks on result:

other: No further details available.

Effect concentrations 2

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

>= 8 g/L

Basis for effect:

growth inhibition

Remarks on result:

other: No further details available.

Details on results:

Up to the maximal water solubility, the test substance is not toxic to Pseudomonas putida and possesses no toxic, water soluble ingredient or impurity.

Results with reference substance (positive control):

Not applicable.

Reported statistics and error estimates:

Neither statistics nor error estimates are reported.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Basic scientific criteria were met.

Conclusions:

The report describes a valid guideline study conducted according to a German national standard procedure (DIN 38 412) under GLP compliance. Basic information available. The test substance is not toxic to Pseudomonas putida (EC10 and EC50 will be equal or greater than 8 g/L).

Executive summary:

The toxic effect on growth of Pseudomonas putida of 1,3,4-Thiadiazolidine-2,5-dithione, reaction products with hydrogen peroxide and tert-dodecanethiol (No CAS, no EC) was investigated according to DIN 38 412 by Hansonis-Jouleh (1993). The test temperature was 21 °C and the test duration 16 h. Test concentrations were used as 80 mg/L and 8000 mg/L (equal to 8 g/L). Cultivation of the pre-culture is done 7 h before inoculation at 21 °C. An aqueous extract is prepared according to DEV S 4 using 100 mg test substance. This amount is mixed with 1 L deionized water and shaked overhead for 24 h. Filtration is done with the help of a rough fluted filter and the filtrate is used for testing. In the second preparation, an eluate with 10 g/L test substance is used. The test is conducted in Erlenmeyer flasks on a shaker with incubation hood. Up to the maximal water solubility, the test substance is not toxic to Pseudomonas putida and possesses no toxic, water soluble ingredient or impurity. Therefore, the EC10(16h) was determined to be >= 8 g/L. The same applies for the EC50(16h) value.