Indium trichloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

briefly documented (only Results tables in English) but acceptable for assessment.

Data source

Reference

Reference Type:

review article or handbook

Title:

Data book for mutagenicity tests on chemicals in bacteria

Author:

Ishidate M

Year:

1991

Bibliographic source:

LIC/Tokyo

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Indium trichloride

EC Number:

233-043-0

EC Name:

Indium trichloride

Cas Number:

10025-82-8

Molecular formula:

Cl3In

IUPAC Name:

indium trichloride

Details on test material:

- Name of test substance: indium trichloride InCl3 (111In)
- CAS No.: 10025-82-8
- Molecular weight: 221.18

Method

Target gene:

not applicable

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium, other: S. typhimurium TA 100, TA 2637, TA 94, TA 98

Species / strain 2

Species / strain / cell type:

E. coli, other: E. coli WP2µvrA+, E. coli WP2µvrA-

Metabolic activation:

with and without

Metabolic activation system:

induced rat liver S9 mix

Test concentrations with justification for top dose:

1.25, 2.5, 5.0, 12.5, 25.0, 50.0 µCi/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: distilled water

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Remarks:

With S9 mix: 2-AA (100 μg/plate, strains TA 100, TA 2637, TA 94, TA 98 and WP2µvrA-. Without S9 mix: AF-2 (0.02 μg/plate, strains TA 100, TA 98 and WP2µvrA-), ICR-191 (5 μg/plate, strain TA 2637), MMC (10 μg/plate, strain TA 94)

Positive control substance:

furylfuramide

mitomycin C

other: 2-AA (2-aminoanthracene), ICR-191 (acridine mutagen)

Details on test system and experimental conditions:

METHOD OF APPLICATION: preincubation

DURATION
- Preincubation period: 20min 37°C

NUMBER OF REPLICATIONS:2

Evaluation criteria:

no information

Statistics:

not available

Results and discussion

Test results

Test results 1

Key result

Species / strain:

S. typhimurium, other: S. typhimurium TA 100, TA 2637, TA 94, TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Test results 2

Key result

Species / strain:

E. coli, other: E. coli WP2µvrA+, E. coli WP2µvrA-

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Additional information on results:

none

Any other information on results incl. tables

none

Applicant's summary and conclusion

Conclusions:

The test material was considered to be non-mutagenic for all the used bacterial strains (Salmonella typhimurium as well as Escherichia coli) with as well as without metabolic activation

Executive summary:

A study was conducted to determine the potential mutagenicity of the test material using bacterial reverse mutation assay (e.g. Ames test). 

No significant increases in the frequency of revertant colonies were recorded at any dose level.

 

The test material was considered to be non-mutagenic under the conditions of this test.