Registration Dossier
Registration Dossier
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EC number: 203-636-9 | CAS number: 108-99-6
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well conducted study by NTP done in compliance with GLP.
Data source
Reference
- Reference Type:
- other: NTP website
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- b-Picoline
- IUPAC Name:
- b-Picoline
- Reference substance name:
- 3-methylpyridine
- EC Number:
- 203-636-9
- EC Name:
- 3-methylpyridine
- Cas Number:
- 108-99-6
- Molecular formula:
- C6H7N
- IUPAC Name:
- 3-methylpyridine
Constituent 1
Constituent 2
Test animals
- Species:
- mouse
- Strain:
- B6C3F1
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: drinking water
- Duration of treatment / exposure:
- 13 week
- Frequency of treatment:
- Daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
78, 156, 312.5, 625 or 1,250 mg/l
Basis:
analytical conc.
- No. of animals per sex per dose:
- 10
- Control animals:
- yes, concurrent no treatment
Examinations
- Tissues and cell types examined:
- Erythrocytes
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION: All doses.
TREATMENT AND SAMPLING TIMES ( in addition to information in specific fields): 0-24 hours
DETAILS OF SLIDE PREPARATION: 1,000 to 10,000 mature erythrocytes (normochromatic erythrocytes or NCEs) are scored per animal for presence
of micronuclei. Acridine orange.
METHOD OF ANALYSIS: Flow cytometry.
OTHER: - Statistics:
- Trend test
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Following a 13-week drinking water study in mice at doses up to 1,250 mg/l , b-picoline was not genotoxic in an erythrocyte miconucleus test.
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