1-chloro-4-[(prop-2-yn-1-yloxy)methyl]benzene

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015-02-20 to 2015-03-20

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Experimental study according to guideline and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Version / remarks:

1997

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)

Version / remarks:

2008

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

BASF SE, Experimental Toxicology and Ecology, 67056 Ludwigshafen, Germany

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-4-[(prop-2-yn-1-yloxy)methyl]benzene

EC Number:

813-130-5

Cas Number:

4039-86-5

Molecular formula:

C10H9ClO

IUPAC Name:

1-chloro-4-[(prop-2-yn-1-yloxy)methyl]benzene

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Metabolic activation:

with and without

Metabolic activation system:

S9 mix from rat livers induced with phenobarbital and β-naphthoflavone

Test concentrations with justification for top dose:

10, 33, 100, 333, 1000, 2550 and 5100 μg/plate standard plate test
3.3, 10, 33, 100, 333 and 1000 μg/plate preincubation test

Vehicle / solvent:

- Vehicle used: DMSO
- Justification for choice of vehicle: Due to the insolubility of the test substance in water, DMSO was used as vehicle, which had been demonstrated to be suitable in bacterial reverse mutation tests and for which historical control data are available.

Controls

Controls 1

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene (2-AA)

Remarks:

with metabolic activation

Controls 2

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)

Remarks:

without metabolic activation, TA 1535, TA 100

Controls 3

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 4-nitro-o-phenylenediamine (NOPD)

Remarks:

without metabolic activation, TA 98

Controls 4

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

9-aminoacridine

Remarks:

(AAC), without metabolic activation, TA 1537

Controls 5

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

4-nitroquinoline-N-oxide

Remarks:

(4-NQO), without metabolic activation, E. coli WP2 uvrA

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation); preincubation

DURATION
- Preincubation period: 20 min
- Exposure duration: 48 - 72 h

NUMBER OF REPLICATIONS: 3

DETERMINATION OF CYTOTOXICITY
- decrease in the number of revertants (factor ≤ 0.6)
- clearing or diminution of the background lawn

Evaluation criteria:

Acceptance criteria
Generally, the experiment was considered valid if the following criteria were met:
- The number of revertant colonies in the negative controls was within the range of the historical negative control data for each tester strain.
- The sterility controls revealed no indication of bacterial contamination.
- The positive control substances both with and without S9 mix induced a distinct increase inthe number of revertant colonies within the range of the historical positive control data or above.
- Fresh bacterial culture containing approximately 10E^9 cells per mL were used.

Assessment criteria
The test substance was considered positive in this assay if the following criteria were met:
- A dose-related and reproducible increase in the number of revertant colonies, i.e. at least doubling (bacteria strains with high spontaneous mutation rate, like TA 98, TA 100 and E.coli WP2 uvrA) or tripling (bacteria strains with low spontaneous mutation rate, like TA 1535 and TA 1537) of the spontaneous mutation rate in at least one tester strain either without S9 mix or after adding a metabolizing system.
A test substance was generally considered non-mutagenic in this test if:
- The number of revertants for all tester strains were within the historical negative control data range under all experimental conditions in at least two experiments carried out independently of each other.

Statistics:

No statistical analysis performed.

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

from about 333 μg/plate onward

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

from about 333 μg/plate onward

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: No test substance precipitation was found with and without S9 mix.

ADDITIONAL INFORMATION ON CYTOTOXICITY:
A bacteriotoxic effect (reduced his- or trp- background growth, decrease in the number of his+ or trp+ revertants) was observed in the standard plate and preincubation test depending on the strain and test conditions from about 333 μg/plate onward.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Experiment 1 (standard plate test):

Dose (µg/plate)	Mean number of revertant colonies/3 replicate plates (± S.D.) with different strains of Salmonella typhimurium and E. coli
	TA1535	TA100	TA1537	TA98	WP2 uvrA
	Results without S9
DMSO	13.3 ± 4.7	96.3 ± 6.8	8 ± 3.6	18.3 ± 3.1	26.7 ± 3.8
33	9.3 ± 0.6	84.3 ± 16.2	9.7 ± 2.3	14.3 ± 4	20 ± 6
100	12.7 ± 2.1	97.7 ± 8.5	9.3 ± 1.2	17.3 ± 2.1	22 ± 3.6
333	13 ± 3.5	100.3 ± 3.1	7.3 ± 2.1	11.7 ± 3.1	21.3 ± 3.8
1000	12.3 ± 2.1	92.3 ± 5	11.7 ± 2.1	22.3 ± 3.8	17.7 ± 8.1
2550	0	8.3 ± 9.7	0	0	13 ± 3.5
5100	0	0	0	0	0
MNNG (5.0)	4360.3 ± 332.7	4114.7 ± 314.4
AAC (100)			891.7 ± 356.9
NOPD (10)				401 ± 11.5
4-NQO (5)					1557.7 ± 72.6
	Results with S9
DMSO	10.7 ± 3.2	100.7 ± 9.9	8.3 ± 0.6	26.7 ± 2.1	30 ± 5.6
33	9.7 ± 2.9	88.7 ± 12.2	11.3 ± 1.5	26 ± 4.4	21.7 ± 4.7
100	10.3 ± 3.2	102 ± 13.5	8.3 ± 2.1	27.3 ± 7.6	25.3 ± 2.1
333	8 ± 2	98 ± 6.9	12 ± 5	32.3 ± 3.1	21.7 ± 3.5
1000	8.7 ± 2.9	87 ± 8.7	9.7 ± 2.1	35 ± 7.2	22 ± 5.3
2550	0	70 ± 6.6	0	12 ± 5.6	13.3 ± 6.4
5100	0	11.7 ± 2.1	0	0	0
2-AA (2.5)	331 ± 12.3	2588 ± 112	167.3 ± 3.2	1924.7 ± 117.1
2-AA (60.0)					93.7 ± 37.6

 

Experiment 2 (standard plate test):

Dose (µg/plate)	Mean number of revertant colonies/3 replicate plates (± S.D.) with different strains of Salmonella typhimurium and E. coli
	TA1535	TA1537	TA98
	Results without S9
DMSO	13 ± 1	12 ± 1	17 ± 4.4
10	13 ± 6	7.3 ± 1.5	17 ± 2
33	12 ± 1.7	9.7 ± 5.5	20.3 ± 3.2
100	10 ± 1.7	9.7 ± 2.1	18.3 ± 0.6
333	12.3 ± 2.1	6.7 ± 4	18.7 ± 4.2
1000	11.7 ± 2.5	5 ± 3.6	23 ± 2.6
2550	0	0	0
MNNG (5.0)	4587 ± 356.3
AAC (100)		1393 ± 109.1
NOPD (10)			465 ± 24.5

 

Experiment 3 (preincubation test):

Dose (µg/plate)	Mean number of revertant colonies/3 replicate plates (± S.D.) with different strains of Salmonella typhimurium and E. coli
	TA1535	TA100	TA1537	TA98	WP2 uvrA
	Results without S9
DMSO	15 ± 3.5	90.3 ± 6.5	6.3 ± 1.2	18 ± 6.2	17.3 ± 4.9
3.3	12.7 ± 2.5	81.7 ± 14.4	9.3 ± 1.4	16.7 ± 6.4	20 ± 7
10	14.3 ± 3.8	93.7 ± 5	5 ± 2.6	19 ± 7.9	19.3 ± 2.5
33	11.7 ± 1.5	92.7 ± 14	6.7 ± 3.2	18.3 ± 3.1	18.3 ± 4.6
100	15 ± 4.6	85.7 ± 2.5	4.3 ± 1.5	19.3 ± 4.5	20.3 ± 2.9
333	10.7 ± 1.5	85.3 ± 3.8	4 ± 1.7	15.3 ± 7.6	18.3 ± 6.4
1000	8.7 ± 1.5	64.3 ± 6.8	1.3 ± 0.6	8 ± 1	10 ± 4.4
MNNG (5.0)	3589.3 ± 142.6	1431.7 ± 414.3
AAC (100)			291.7 ± 3.2
NOPD (10)				453 ± 29.8
4-NQO (5)					272.3 ± 52.5
	Results with S9
DMSO	14.3 ± 1.2	96 ± 8.7	7 ± 3	21.7 ± 2.5	18.3 ± 5
3.3	12 ± 1.7	87 ± 8.5	7.7 ± 2.1	25.3 ± 1.5	19 ± 7.2
10	10.3 ± 2.1	95.3 ± 11.6	7.7 ± 2.5	25.3 ± 5.7	15.3 ± 3.1
33	10.7 ± 3.8	86.7 ± 4.5	7 ± 2.6	20.3 ± 4.5	21.3 ± 8.7
100	15.7 ± 3.6	83.3 ± 7	10.7 ± 0.6	25.7 ± 4	22.3 ± 5.1
333	7.7 ± 2.1	70.7 ± 2.3	6.3 ± 4	21.3 ± 2.3	13 ± 6.6
1000	0	48 ± 24.3	1 ± 1.7	1.3 ± 0.6	11.7 ± 0.6
2-AA (2.5)	280.3 ± 19	1791 ± 163.7	124.3 ± 37.9	1382.7 ± 48.2
2-AA (60.0)					156 ± 25.4

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative