1-chloro-4-[(prop-2-yn-1-yloxy)methyl]benzene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

2014-12-09 to 2015-03-23

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Experimental study according to guideline and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)

Version / remarks:

2013

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.46 (In Vitro Skin Irritation: Reconstructed Human Epidermis Model Test)

Version / remarks:

2012

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

BASF SE, Experimental Toxicology and Ecology, 67056 Ludwigshafen, Germany

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-4-[(prop-2-yn-1-yloxy)methyl]benzene

EC Number:

813-130-5

Cas Number:

4039-86-5

Molecular formula:

C10H9ClO

IUPAC Name:

1-chloro-4-[(prop-2-yn-1-yloxy)methyl]benzene

Test animals

Species:

human

Details on test animals or test system and environmental conditions:

Kits used:
- EpiDerm™ 200 kit (Lot: 21607)
- Source: MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia,
- containing: 24 Epi-200 tissues (reconstructed epidermis): surface 0.6 cm² cultured in Millicells® ∅ 1 cm
- Tissue for MTT reduction control: Epi-200 tissue that is killed by freezing at –20°C

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: negative, positive and MTT-reduction control

Amount / concentration applied:

TEST MATERIAL
- Amount applied: 30 µL
- Concentration: undiluted

Duration of treatment / exposure:

1 h

Details on study design:

please refer to "Any other information on materials and methods"

Results and discussion

In vitro

Results

Irritation / corrosion parameter:

other: other: viability

Value:

40.3

Remarks on result:

other:

Remarks:

Basis: mean. Time point: 1 h exposure, 42 h post-exposure. Reversibility: other: not applicable. Remarks: %. (migrated information)

Any other information on results incl. tables

Test substance			Tissue 1	Tissue 2	Tissue 3	Mean	SD	CV [%]
Negative control	Viable tissues	Mean OD570	2.304	2.649	2.488	2.480	0.173
		Viability [% of NC]	92.9	106.8	100.3	100.0	7.0	7.0
	KC tissues	Mean OD570	0.062	0.066	0.055	0.061	0.005
		Viability [% of NC]	2.5	2.6	2.2	2.4	0.2	8.7
Test substance	Viable tissues	Mean OD570	0.812	0.986	1.198	0.999	0.193
		Viability [% of NC]	32.7	39.8	48.3	40.3	7.8	19.4
	KC tissues	Mean OD570 corrected	0	0	0	0	0
		Viability [% of NC]	0	0	0	0	0	-
Positive control	Viable tissues	Mean OD570	0.063	0.068	0.072	0.067	0.005
		Viability [% of NC]	2.5	2.7	2.9	2.7	0.2	7.1

Negative values were set to zero for further calculation

CV=coefficient of variation

SD=standard deviation

Due to the ability of the test substance to reduce MTT directly, KC tissues were applied in parallel. However, the result of the KC did not indicate an increased MTT reduction. Thus the mean viability is given without KC correction.

Applicant's summary and conclusion

Interpretation of results:

irritating

Remarks:

Migrated information