Methyl anthranilate

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

accepted calculation method

Justification for type of information:

Data is from modelling database

Qualifier:

according to guideline

Guideline:

other: Modeling database

Principles of method if other than guideline:

ECOSAR v1.1

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

-Common name: Water Flea

Test type:

not specified

Water media type:

not specified

Total exposure duration:

21 d

Key result

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

4.36 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: other details not available

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of EPI suite, ECOSAR version 1.11, the EC 50 value for long term toxicity to aquatic invertebrates was predicted to be 4.36mg/l for test chemical in 21 days.
Based on this value it can be concluded that the substance was considered to be nontoxic to aquatic environment and cannot be classified as per the criteria mentioned in CLP regulation.

Executive summary:

Based on the prediction done by EPI suite, ECOSAR version 1.11, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the EC 50 value for long term toxicity to aquatic invertebrates was predicted.

On the basis of this program, the EC 50 value for long term toxicity to aquatic invertebrates was predicted to be 4.36 mg/l for test chemical in 21 days. Based on this value it can be concluded that the substance was considered to be nontoxic to aquatic environment and cannot be classified as per the criteria mentioned in CLP regulation.

Description of key information

Based on the prediction done by EPI suite, ECOSAR version 1.11, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the EC 50 value for long term toxicity to aquatic invertebrates was predicted.

On the basis of this program, the EC 50 value for long term toxicity to aquatic invertebrates was predicted to be 4.36 mg/l for test chemical in 21 days. Based on this value it can be concluded that the substance was considered to be nontoxic to aquatic environment and cannot be classified as per the criteria mentioned in CLP regulation.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

4.36 mg/L

Additional information

Predicted study for target chemical and experimental study for its structurally similar read across chemical have been reviewed to determine the long term toxicity of test chemical to aquatic invertebrates and their results are summarized below.

The first study based on the prediction done by EPI suite, ECOSAR version 1.11, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the EC 50 value for long term toxicity to aquatic invertebrates was predicted. On the basis of this program, the EC 50 value for long term toxicity to aquatic invertebrates was predicted to be 4.36 mg/l for test chemical in 21 days. Based on this value it can be concluded that the substance was considered to be nontoxic to aquatic environment and cannot be classified as per the criteria mentioned in CLP regulation.

Next study reviewed from journal water Research in this An experiment was conducted to determine the long term toxicity of test chemical aquatic invertebrates using DIN-Standard 38412, Part II guideline for 21 days reproduction test. The test organism used in this study was Daphnia magna ((IRCHA strain). The stock solution of test chemical of concentration 400 mg/L was prepared by stirring test chemical in water for 1 h. The nominal concentration of test chemical used were in range of 0.4-100 mg/L. The experiment was conducted in semi-static condition using fresh water (Berlin tap water) in 2 litre capacity beaker and it was covered by watch glass, the number of organism per vessel were 20-30.The test chemical solutions of different nominal concentrations were exposed Daphnia manga for 21 days in replicates at temperature 20°C and pH 7.6-7.7 the test solution was oxygen saturated. The analytical monitoring of test chemical solution was done by using chemical method. After 21 days of exposure of test chemical to Daphnia magna the EC50 value and NOEC value was determined to be 7 mg/L and 13 mg/L. 

Last study was reviewed from authoritative database in this an experiment was conducted to determine the long term toxicity of test chemical aquatic invertebrates OECD Guideline 211 (Daphnia magna Reproduction Test). The test organism used in this study was Daphnia magna. The experiment was conducted in semi-static condition using fresh water. The test chemical solutions of different nominal concentrations were exposed Daphnia manga for 21 days. After 21 days of exposure of test chemical to Daphnia magna the EC50 value and NOEC value was determined to be 1.2 mg/L and 0.71 mg/L. 

By considering results of all the study mentioned above the EC50 value of test chemical was determined to be in range of 1.2 to 13 mg/L after exposure of test chemical for 21 days to daphnia magna. By considering range EC50 value it is concluded that test chemical non-toxic to aquatic organisms and cannot be classified as per CLP regulation