1,2,3-trichlorobenzene

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Version / remarks:

1981

Deviations:

yes

Remarks:

Analytical purity not reported. No GLP

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,2,3-trichlorobenzene

EC Number:

201-757-1

EC Name:

1,2,3-trichlorobenzene

Cas Number:

87-61-6

Molecular formula:

C6H3Cl3

IUPAC Name:

1,2,3-trichlorobenzene

Test material form:

solid

Details on test material:

- Name of test material (as cited in study report): 1,2,3-trichlorobenzene (solidified melted)
- Physical state: solid
- Analytical purity: no data

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Hacking & Churchill ltd., Huntingdon, UK
- Weight at study initiation: 3 kg
- Diet (e.g. ad libitum): Feed "Ssniff K4" (Ssniff Versuchstierdiaten GmbH, Soest/Westf., Germany) ad libitum
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-21°C Celsius
- Humidity (%): 40-50%
- Photoperiod (hrs dark / hrs light): 12 hrs dark / 12 hrs ligh (7 a.m.-7 p.m.)

IN-LIFE DATES: The test was conducted during April 1982

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: other eye served as control

Amount / concentration applied:

Amount applied: 100 mg

Duration of treatment / exposure:

24 hour(s)

Observation period (in vivo):

1, 24, 48, 72 hours, and 7 days

Number of animals or in vitro replicates:

3

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): physiological solution
- Time after start of exposure: 24 hours after exposure

SCORING SYSTEM: according to Draize method

TOOL USED TO ASSESS SCORE: fluorescein

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0.7

Max. score:

4

Reversibility:

fully reversible within: 72 hrs

Results 2

Irritation parameter:

cornea opacity score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

4

Results 3

Irritation parameter:

cornea opacity score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0.7

Max. score:

4

Reversibility:

fully reversible within: 72 hrs

Results 4

Irritation parameter:

iris score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0

Max. score:

2

Results 5

Irritation parameter:

iris score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

2

Results 6

Irritation parameter:

iris score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0

Max. score:

2

Results 7

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

24/48/72 h

Score:

1.3

Max. score:

3

Reversibility:

fully reversible within: 72 hrs

Results 8

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0.7

Max. score:

3

Reversibility:

fully reversible within: 72 hrs

Results 9

Irritation parameter:

conjunctivae score

Basis:

animal #3

Time point:

24/48/72 h

Score:

1.3

Max. score:

3

Reversibility:

fully reversible within: 72 hrs

Results 10

Irritation parameter:

chemosis score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0.3

Max. score:

4

Reversibility:

fully reversible within: 48 hrs

Results 11

Irritation parameter:

chemosis score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

4

Results 12

Irritation parameter:

chemosis score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0.7

Max. score:

4

Reversibility:

fully reversible within: 72 hrs

Irritant / corrosive response data:

All observed symptoms were completely away 72 hours after application.
Additional symptoms were not observed.

Any other information on results incl. tables

Readings	Animal	Cornea	Iris	Conjunctiva Redness	Conjuctiva Chemosis	Discharge
24h	41	1	0	3	1	1
24h	42	0	0	1	0	0
24h	43	1	0	3	1	2
48h	41	1	0	1	0	0
48h	42	0	0	1	0	0
48h	43	1	0	1	1	0
72h	41	0	0	0	0	0
72h	42	0	0	0	0	0
72h	43	0	0	0	0	0
Mean24-72h	41	0.7	0	1.3	0.3	0
Mean24-72h	42	0	0	0.7	0	0.3
Mean24-72h	43	0.7	0	1.3	0.7	0.7

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Mean (24/48/72 hrs) scores per animal:
- Conjunctivae redness: 1.3 / 0.7 / 0.7;
- Cornea opacity: 0.7 / 0.0 0.7;
- Iritis: 0.0 / 0.0 /0.0;
- Conjunctival oedema (chemosis): 0.3 / 0.0; 0.7.

Executive summary:

Suberg H (1982)

An acute eye irritation/corrosion study was conducted according OECD Guideline 405 with deviations (Analytical purity not reported. No GLP).

3 New Zealand White rabbits were exposed to 100 mg 1,2,3 -trichlorobenzene for 24 hours. Observation time points were 1h, 24h, 48, 72h and 7 days after removal of the patch.

Mean (24/48/72 hrs) scores per animal:
- Conjunctivae redness: 1.3 / 0.7 / 0.7;
- Cornea opacity: 0.7 / 0.0 0.7;
- Iritis: 0.0 / 0.0 /0.0;
- Conjunctival oedema (chemosis): 0.3 / 0.0; 0.7.
Within 72 hour reading all animals had recovered.

On the basis of the data obtained 1,2,3-trichlorobenzene can not be classified as irritant.