3-methyl-2-pent-2-enylcyclopent-2-enone

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study done to guideline, in compliance with GLP

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Nominal and measured concentrations:

Nominal: 10, 18, 32, 56 and 100 mg/l.
Average Measured: 9.7, 19, 39, 58 and 110 mg/l.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

45 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence interval ranging from 40-49 mg/L

Details on results:

The actual concentrations measured at the start of the test were at the level of 98-135% of nominal and remained stable during the exposure (80-103% of initial at the end of the test).
Because the actual concentration at 32 mg/l deviated more than 20% from the nominal concentration the average exposure concentrations were calculated and the effect parameters were expressed in terms of average exposure concentration.

Under the conditions of the present study the 48h-EC50 was 45 mg/l based on average exposure concentrations, with a 95% confidence interval ranging from 40-49 mg/l.

Validity criteria fulfilled:

yes

Executive summary:

Under the conditions of the present study the 48h-EC50 was 45 mg/l based on average exposure concentrations, with a 95% confidence interval ranging from 40-49 mg/l.

Description of key information

EC50 (48 h) = 45.0 mg/L (geometric mean measured, OECD 202, D. magna)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

45 mg/L

Additional information

There is one study available, in which the short-term toxicity of (Z)-3-methyl-2-(pent-2-en-1-yl)cyclopent-2-enone (CAS 488-10-8) to aquatic invertebrates was assessed according to the OECD guideline 202 and GLP.

In a static test, Daphnia magna was exposed to the nominal concentrations of 10, 18, 32, 56 and 100 mg/L test item for 48 h. The test item concentrations were analytically verified by GC at the start and end of the test.

The measured test item concentrations at the start of the test were 98 – 135% of nominal and remained stable throughout the exposure (80 – 103% of initial at test end). Because the actual concentration at 32 mg/L deviated more than 20% from the nominal concentration the average exposure concentrations were calculated and the effect parameters were expressed in terms of average exposure concentration. The average exposure concentrations were 9.7, 19, 39, 58 and 110 mg/l, respectively. After 48 h immobilization was observed and resulted in an EC50 (48 h) of 45 mg/L based on average exposure concentrations, with a 95% confidence interval ranging from 40-49 mg/L.