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EC number: 224-208-8 | CAS number: 4247-02-3
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
ErC50 (72 h) = 8.5 mg/L for Desmodesmus subspicatus (OECD 201, full test)
ErC10 (72 h) = 4.07 mg/L for Desmodesmus subspicatus (OECD 201, full test)
NOErC (72 h) = 1.25 mg/L for Desmodesmus subspicatus (OECD 201, full test)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 8.5 mg/L
- EC10 or NOEC for freshwater algae:
- 4.07 mg/L
Additional information
In the acute and chronic test with the unicellular freshwater green alga Desmodesmus subspicatus (Scheerbaum, 2016b), the effects of the test item isobutyl 4-hydroxybenzoate (CAS No. 4247-02-3) towards green algae were determined according to OECD 201 (2011) and GLP. The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours. The study was conducted under static conditions. Based on a preliminary range finding test a stock solution with a nominal concentration of 100 mg/L was prepared in dilution water. The dispersion was stirred for 2 hours with approximately 1100 rpm at room temperature. After the stirring period the stock solution was treated with ultrasound for 10 minutes until the test item was completely dissolved and thereafter diluted to a stock solution of 20 mg/L. Five concentration levels out of the stock solution (20 mg/L) were tested in a geometrical series: 1.25 - 2.50 - 5.00 - 10.0 - 20.0 mg/L. The concentrations of the test item in all test concentrations and the control were analytically verified by HPLC-DAD at the start and the end of the exposure. The measured concentrations in the fresh media (0 h) were in the range of 97 to 100% of the nominal values in all tested concentration levels. The measured concentrations in the old media (72 h) were in the range of 91 to 101% of the nominal values in all tested concentration levels. Therefore, all effect values given are based on the nominal test item concentrations. An ErC50 (72 h) of 8.5, mg/L, an ErC10 (72 h) of 4.07 mg/L, and a NOErC (72 h) of 1.25 mg/L were determined.
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