Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 224-208-8 | CAS number: 4247-02-3
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
EC50 (48 h) = 5.3 mg/L for Daphnia magna (OECD 202, full test)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 5.3 mg/L
Additional information
In the acute immobilisation test with Daphnia magna (Scheerbaum, 2016a), the effects of the test item isobutyl 4-hydroxybenzoate (CAS No. 4247-02-3) were determined according to OECD 202 (2004) guideline and GLP. The study was conducted under static conditions over a period of 48 hours with 5 concentrations in the range of 0.625 - 10.0 mg/L. Twenty daphnids were exposed to each concentration level and the control. The concentrations of the test item were analytically verified via HPLC-DAD at the start and at the end of the exposure in all concentration levels and in the control. The measured concentrations of the test item were in the range of 93 to 98% of the nominal values at the start and at the end of the exposure. The measured test item concentrations were all within ±20% of the nominal concentrations. This indicates that the test item concentrations were successfully maintained for the duration of the test. Therefore, the effect levels are based on the nominal concentrations. An EC50 (48 h) of 5.3 mg/L was determined.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
