Disodium [4-hydroxy-3-[(2-hydroxy-4-nitrophenyl)azo]naphthalene-1-sulphonato(3-)][1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)]chromate(2-)

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

yes

Remarks:

Mean of 2 determinations instead of 3.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Code number: FAT-20011/D
Batch-Nr: 61
Purity: ca 52.5 %
Appearance: solid
Solubility: miscible
Storage: room temperature
Expiration date: 07/99

Oxygen conditions:

aerobic

Inoculum or test system:

other: titration method

Details on inoculum:

None

Duration of test (contact time):

2 h

Initial conc.:

10 other: mg

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Experimental conditions:
- Temperature: 148 +/- 3 °C
- Duration: 2 hours
- Estimation Technique: Titration (potentiometric), METROHM Titroprozessor 670 (SAV: 00 111)

Reference substance:

other: Pottassium hydrogen phtalate solution (Merck-Nr. 11 769)

Test performance:

The COD of Potassium hydrogen phthalate is 198 mg O2/I (Validity: 200 ± 8 mg 02/l)

Remarks on result:

not measured/tested

Details on results:

None

Parameter:

COD

Value:

496 mg O2/g test mat.

Results with reference substance:

The COD of Potassium hydrogen phthalate was found to be 198 mg O2/I (Validity: 200 ± 8 mg 02/l)

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The COD of the test substance FAT 20011/D is 496 mg O2/g.

Executive summary:

A predetermined amount of the test substance FAT 20011/D dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. This test was carried out according to DEV DIN 38*409 - H 41-1 (1980) corresponding to 92/96/EEC C.6. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland.

The test substance was tested with a sample weight of 10 mg. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/l. The COD of the reference substance was found to be: 198 mg O2/l

Result:

Based on the findings of this study, the COD of FAT 20011/D is 496 mg O2/g. In another experiment, BOD of FAT 20011/D was found to be 4 mg O2/g. The resultant BOD/COD ratio was found to be 0.008. FAT 20011/D was thus considered to be not biodegradable.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.5 (Degradation: Biochemical Oxygen Demand)

Version / remarks:

ISO 5815 Second Edition 1989-08-01 (E) corresponding to 92/96/EEC C.5

Deviations:

yes

Remarks:

Aerated over night after addition of the inoculum

GLP compliance:

yes

Specific details on test material used for the study:

Code number: FAT - 20'011/D
Batch-Nr: 61
Purity ca: 52.5 %
Appearance: solid
Solubility: miscible
Storage: room temperature
Expiration date: 07/99

Oxygen conditions:

aerobic

Inoculum or test system:

other: Seeding water of an urban waste water

Duration of test (contact time):

5 d

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Experimental conditions:
- Test concentrations: About 6 to 800 mg test substance/litre dilution water
- Temperature: 20 +/- 1 °C
- Duration: 5 days
- Estimation Technique: O2 - Determination measured with an oxygen sensitive electrode system: SYLAND (SAV: 00 107)
- Suppression of nitrification: without suppression of nitrification.

Reference substance:

other: D (+) - Glucose (Merck Nr. 8337) / L-Glutamic acid (Merck N°291)

Parameter:

other: BOD

Remarks on result:

other: BOD determination

Parameter:

BOD5

Value:

4 mg O2/g test mat.

Results with reference substance:

The BOD5 of Glucose/Glutamic acid solution is 207 mg O2/l.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The BOD5 of FAT 20011/D corrected by the blank control is 4 mg O2/g.

Executive summary:

The biological oxygen demand of FAT 20011/D is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of microorganisms. The test was carried out according to ISO 5815 Second edition 1989 -08 -01 (E) corresponding to 92 /96/EEC C.5. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland.

The test was tested in concentrations from about 6 to 800 mg/l. The reference substance D(+)-Glucose / L-Glutamic acid was tested in a concentration of 20ml/l. The BOD5 of Glucose/Glutamic acid solution is 207 mg O2/l.

Based on the findings of the study, the BOD5 of FAT 20011/D corrected by the blank control is 4 mg O2/g. Considering the COD of FAT 20011/D which was 496 mg O2/g, the BOD/COD ratio was found to be 0.008. The substance was thus considered as not biodegradable.

Reference 3

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: EEC-Directive, L133 (pp. 99-105) Volume 31, May 88.

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Code number: FAT 20011/D
Batch-Nr: 61
Purity: ca 52.5 %
Appearance: solid
Solubility: miscible
Storage: room temperature
Expiration date: 07/99

Oxygen conditions:

aerobic

Inoculum or test system:

other: Sludge of a biological sewage treatment plant.

Details on inoculum:

Sludge of a biological sewage treatment plant (ARA-Reinach) collected on 19.04.1994

Duration of test (contact time):

28 d

Initial conc.:

150 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

Experimental conditions
Water / Nutrient - according to the specification of the EEC L133 (pp. 99-105) Volume 31 resp. OECD Method 302B.
Temperature: 22.0±3 °C (room temperature)
Test duration - 28 days
Lighting - indirect daylight
Estimation Technique - TOC/DOC Analyzer/ SHIMADZU TOC-5000

Reference substance:

diethylene glycol

Test performance:

The bioelimination, corrected by the blank control and measured as DOC (mg/l) is: 99.7 % 14th day

Parameter:

% degradation (DOC removal)

Value:

22.9

Sampling time:

28 d

Results with reference substance:

The bioelimination of the reference substance after 14 days is 99. 7%.

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

The bioelimination of the test substance FAT 20011/D after 28 days is: 22.9 %.

Executive summary:

The inherent biodegradability of FAT 20011/D was determined in a 28 days Zahn-Wellens - Test according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1981 and the EEC-Directive, L133 (pp. 99-105) Volume 31, May 88. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The test substance was tested in concentration of 150 mg/l DOC.

 

The reference substance Diethylenglycol was also tested in a concentration of 150 mg/l DOC.

 

Based on the findings of the study, the bioelimination of the test substance FAT - 20'011/D after 28 days is: 22.9 %.

Description of key information

The BOD5 of FAT 20011/D corrected by the blank control is 4 mg O2/g. Considering the COD of FAT 20011/D which was 496 mg O2/g, the BOD/COD ratio was found to be 0.008. The substance was thus considered as not rapidly biodegradable. In several supporting studies, following BOD and COD values were found:

BOD5 of FAT 20011/A: 180 mg/g O2

COD of FAT 20011/A: 1018.07 mg O2/g test material.

BOD5 of FAT 20011/B: 574.79 mg/g O2

COD of FAT 20011/B: 33 mg O2/g test material.

BOD5 of FAT 20011/C: 20 mg/g O2

COD of FAT 20011/C: 1277.7 mg O2/g test material.

The BOD5/COD quotient from all the above studies <0.05, therefore the substance is not indicative of rapid degradation.

In a Zahn-Wellens test conducted according to OECD Guideline 302B, the bioelimination of the test substance FAT - 20'011/D after 28 days was 22.9 %. Taking the data from all the studies into account, Acid Blue 317 is considered to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Type of water:

freshwater